Three new potential ovarian cancer biomarkers detected in human urine with equalizer bead technology
| Autor: | Eric T. Fung, Claus Høgdall, Tai-Tung Yip, Anja Hviid Simonsen, Lene Lundvall, Estrid Høgdall, Anette Lykke Petri |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Proteomics Pathology medicine.medical_specialty Pelvic mass Urology Equalizer Urine Mass spectrometry Tandem mass spectrometry Risk Assessment Sensitivity and Specificity Sampling Studies Statistics Nonparametric Predictive Value of Tests Biomarkers Tumor medicine Humans Electrophoresis Gel Two-Dimensional Prospective Studies Aged Neoplasm Staging Pelvic Neoplasms Probability Aged 80 and over Ovarian Neoplasms Laparotomy business.industry Biopsy Needle Obstetrics and Gynecology General Medicine Middle Aged Prognosis medicine.disease Immunohistochemistry ROC Curve Area Under Curve CA-125 Antigen Disease Progression Biomarker (medicine) Female business Ovarian cancer |
| Zdroj: | Acta Obstetricia et Gynecologica Scandinavica. 88:18-26 |
| ISSN: | 1600-0412 0001-6349 |
| DOI: | 10.1080/00016340802443830 |
| Popis: | To examine whether urine can be used to measure specific ovarian cancer proteomic profiles and whether one peak alone or in combination with other peaks or CA125 has the sensitivity and specificity to discriminate between ovarian cancer pelvic mass and benign pelvic mass.A total of 209 women were admitted for surgery for pelvic mass at the Gynaecological Department at Rigshospitalet, Copenhagen. Of the women, 156 had benign gynaecological tumors, 13 had borderline tumors and 40 had malignant epithelial ovarian cancer. The prospectively and preoperatively collected urine samples were aliquotted and frozen at -80 degrees until the time of analysis. The urine was fractionated using equalizer bead technology and then analyzed with surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Biomarkers were purified and identified using combinations of chromatographic techniques and tandem mass spectrometry.Benign and malignant ovarian cancer cases were compared; 21 significantly different peaks (p0.001) were visualized using Mann-Whitney analysis, ranging in m/z values from 1,500 to 185,000. The three most significant peaks were purified and identified as fibrinogen alpha fragment (m/z=2570.21), collagen alpha 1 (III) fragment (m/z=2707.32) and fibrinogen beta NT fragment (m/z=4425.09). The area under the receiver operator characteristic curve (ROC AUC) value for these three peaks in combination was 0.88, and their ROC AUC value in combination with CA125 was 0.96.This result supports the feasibility of using urine as a clinical diagnostic medium, and the ROC AUC value for the three most significant peaks in combination with or without CA125 demonstrates the enhanced prediction performance of combined marker analysis. |
| Databáze: | OpenAIRE |
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