Virulence-related genes, adhesion and invasion of some Yersinia enterocolitica-like strains suggests its pathogenic potential
| Autor: | Roberto A. Souza, Jaqueline Passaglia, Juliana Pfrimer Falcão, Priscilla F. M. Imori, Lenaldo B. Rocha |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Yersinia Infections Virulence Factors Highly pathogenic 030106 microbiology Virulence Biology Microbiology Bacterial Adhesion Cell Line 03 medical and health sciences FepA Humans Yersinia enterocolitica Gene Cells Cultured Sequence Analysis DNA Adhesion Pathogenicity biology.organism_classification 030104 developmental biology Infectious Diseases Genes Bacterial |
| Zdroj: | Microbial Pathogenesis. 104:72-77 |
| ISSN: | 0882-4010 |
| Popis: | Yersina enterocolitica-like species have not been extensively studied regarding its pathogenic potential. This work aimed to assess the pathogenic potential of some Y. enterocolitica-like strains by evaluating the presence of virulence-related genes by PCR and their ability to adhere to and invade Caco-2 and HEp-2 cells. A total of 50 Y. frederiksenii, 55 Y. intermedia and 13 Y. kristensenii strains were studied. The strains contained the following genes: Y. frederiksenii, fepA(44%), fes(44%) and ystB(18%); Y. intermedia, ail(53%), fepA (35%), fepD(2%), fes(97%), hreP(2%), ystB(2%) and tccC(35%); Y. kristensenii, ail(62%), ystB(23%), fepA(77%), fepD(54%), fes(54%) and hreP(77%). Generally, the Y. enterocolitica-like strains had a reduced ability to adhere to and invade mammalian cells compared to the highly pathogenic Y. enterocolitica 8081. However, Y. kristensenii FCF410 and Y. frederiksenii FCF461 presented high invasion potentials in Caco-2 cells after five days of pre-incubation increased by 45- and 7.2-fold compared to Y. enterocolitica 8081, respectively; but, the ail gene was not detected in these strains. The presence of virulence-related genes in some of the Y. enterocolitica-like strains indicated their possible pathogenic potential. Moreover, the results suggest the existence of alternative virulence mechanisms and that the pathogenicity of Y. kristensenii and Y. frederiksenii may be strain-dependent. |
| Databáze: | OpenAIRE |
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