CYP26B1 declines postnatally in Sertoli cells independently of androgen action in the mouse testis
| Autor: | Nadia Yasmín Edelsztein, Helena Fedora Schteingart, Kenichi Kashimada, Rodolfo Rey |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male endocrine system medicine.drug_class Period (gene) Retinoic acid Down-Regulation Tretinoin Biology Transfection Cell Line Andrology 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Meiosis Pregnancy Genetics medicine Animals Gonads 030219 obstetrics & reproductive medicine Binding Sites Sertoli Cells urogenital system Gene Expression Regulation Developmental Cell Biology Retinoic Acid 4-Hydroxylase Androgen Sertoli cell Prenatal development Androgen receptor 030104 developmental biology medicine.anatomical_structure chemistry Animals Newborn Receptors Androgen Androgens Immunohistochemistry Female Developmental Biology Signal Transduction |
| Zdroj: | Molecular reproduction and developmentREFERENCES. 87(1) |
| ISSN: | 1098-2795 |
| Popis: | Meiosis begins at puberty and relies on several factors, including androgens and retinoic acid in the mouse testis. CYP26B1 degrades retinoic acid in the testis during prenatal development preventing meiosis initiation. Given the concurrence of meiotic entry and completion of Sertoli cell maturation in response to androgens at puberty in the mouse, we proposed that CYP26B1 is downregulated by androgens in the Sertoli cell during this period. By immunohistochemistry, we showed that CYP26B1 declines in Sertoli cells after birth. However, luciferase reporter assays and quantitative reverse transcription-polymerase chain reaction performed in the prepubertal mouse Sertoli cell line SMAT1 revealed no changes in Cyp26b1 expression in response to androgen treatment. Furthermore, studies carried out using primary Sertoli cells of 10-day-old mice showed no changes in either Cyp26b1 or CYP26B1 expression in response to androgen treatment. In summary, the hereby reported decline in CYP26B1 expression in Sertoli cells towards pubertal onset does not appear to be caused by a direct inhibitory effect of androgens on Sertoli cells in the mouse. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text