Red pulp macrophages in the human spleen are a distinct cell population with a unique expression of Fc-γ receptors
| Autor: | Christine W. Bruggeman, Sietse Q. Nagelkerke, Joke M. M. den Haan, Robin van Bruggen, Erik Mul, Taco W. Kuijpers, Timo K. van den Berg |
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| Přispěvatelé: | Landsteiner Laboratory, Paediatric Infectious Diseases / Rheumatology / Immunology, ARD - Amsterdam Reproduction and Development, Molecular cell biology and Immunology, AGEM - Digestive immunity, APH - Aging & Later Life, Pediatric surgery, Amsterdam Reproduction & Development (AR&D) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Population Spleen Immunoglobulin G 03 medical and health sciences Phagocytes Granulocytes and Myelopoiesis stomatognathic system Phagocytosis medicine Humans education Receptor education.field_of_study biology Chemistry Macrophages Receptors IgG Hematology medicine.disease Molecular biology stomatognathic diseases 030104 developmental biology medicine.anatomical_structure Splenic Red Pulp biology.protein Red pulp Antibody Autoimmune hemolytic anemia |
| Zdroj: | Blood advances, 2(8), 941-953. American Society of Hematology Nagelkerke, S Q, Bruggeman, C W, den Haan, J M M, Mul, E P J, van den Berg, T K, van Bruggen, R & Kuijpers, T W 2018, ' Red pulp macrophages in the human spleen are a distinct cell population with a unique expression of Fc-γ receptors ', BLOOD ADVANCES, vol. 2, no. 8, pp. 941-953 . https://doi.org/10.1182/bloodadvances.2017015008, https://doi.org/10.1182/bloodadvances.2017015008 BLOOD ADVANCES, 2(8), 941-953. American Society of Hematology |
| ISSN: | 2473-9529 |
| Popis: | Tissue-resident macrophages in the spleen play a major role in the clearance of immunoglobulin G (IgG)-opsonized blood cells, as occurs in immune thrombocytopenia (ITP) and autoimmune hemolytic anemia (AIHA). Blood cells are phagocytosed via the Fc-γ receptors (FcγRs), but little is known about the FcγR expression on splenic red pulp macrophages in humans, with only a few previous studies that showed conflicting results. We developed a novel method to specifically isolate red pulp macrophages from 82 human spleens. Surface expression of various receptors and phagocytic capacity was analyzed by flow cytometry and immunofluorescence of tissue sections. Red pulp macrophages were distinct from splenic monocytes and blood monocyte-derived macrophages on various surface markers. Human red pulp macrophages predominantly expressed the low-affinity receptors FcγRIIa and FcγRIIIa. In contrast to blood monocyte-derived macrophages, red pulp macrophages did not express the inhibitory FcγRIIb. Red pulp macrophages expressed very low levels of the high-affinity receptor FcγRI. Messenger RNA transcript analysis confirmed this expression pattern. Unexpectedly and despite these differences in FcγR expression, phagocytosis of IgG-opsonized blood cells by red pulp macrophages was dependent on the same FcγRs as phagocytosis by blood monocyte-derived macrophages, especially in regarding the response to IV immunoglobulin. Concluding, we show the distinct nature of splenic red pulp macrophages in human subjects. Knowledge on the FcγR expression and usage of these cells is important for understanding and improving treatment strategies for autoimmune diseases such as ITP and AIHA. |
| Databáze: | OpenAIRE |
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