Antigenotoxic and antimutagenic effects of diphenyl ditelluride against several known mutagens in Chinese hamster lung fibroblasts
| Autor: | Nathalia Rammé Medeiros de Albuquerque, João Antonio Pêgas Henriques, Iuri Marques de Oliveira, André Luiz Mendes Juchem, Temenouga N. Guecheva, Renato Moreira Rosa, Cristiano Trindade, Jenifer Saffi |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
DNA damage
Thiobarbituric acid Health Toxicology and Mutagenesis Toxicology Chinese hamster Cell Line Superoxide dismutase chemistry.chemical_compound Cricetinae Benzene Derivatives Organometallic Compounds Genetics TBARS Animals Lung Micronuclei Chromosome-Defective Genetics (clinical) L-Lactate Dehydrogenase biology Mutagenicity Tests Superoxide Dismutase Chemistry Diphenyl ditelluride Antimutagenic Agents Fibroblasts Catalase biology.organism_classification Comet assay Oxidative Stress Biochemistry biology.protein Comet Assay Lipid Peroxidation Reactive Oxygen Species Micronucleus Biomarkers Mutagens |
| Zdroj: | Mutagenesis. 30:799-809 |
| ISSN: | 1464-3804 0267-8357 |
| Popis: | The present study evaluates antigenotoxic and antimutagenic properties of diphenyl ditelluride (DPDT) against several known mutagens in Chinese hamster lung fibroblasts (V79 cells). DPDT was not cytotoxic and genotoxic at concentrations ranging from 0.01 to 0.1 μM. The pre-treatment for 2h with this organotellurium compound at non-cytotoxic dose range (0.01, 0.05 and 0.1 μM) increased cell survival after challenge with hydrogen peroxide (H2O2), t-butyl hydroperoxide (t-BOOH), methylmethanesulphonate (MMS) or ultraviolet (UV)C radiation. In addition, the pre-treatment with DPDT decreased the DNA damage and Formamidopyrimidine DNA-glycosylase (Fpg)- and Endonuclease III (Endo III) sensitive sites induction by the studied genotoxic agents, as verified by comet assay and modified comet assay, respectively. The pre-treatment also reduced micronucleus frequency, revealing the protector effect of DPDT against MMS and UVC-induced mutagenesis. Our results demonstrate that DPDT-treated cells at concentration range of 0.01-0.1 μM do not change thiobarbituric acid reactive species (TBARS) levels and ROS generation. Moreover, DPDT pre-treatment at this concentration range decreases the ROS induction by H2O2 and t-BOOH treatment indicating antioxidant potential. On the other hand, concentrations higher than 0.1 μM increase TBARS formation and inhibited superoxide dismutase (SOD) activity, suggesting pro-oxidative effect of this compound at high concentrations. Our results suggest that DPDT presents antigenotoxic and antimutagenic properties at concentration range of 0.01-0.1 μM. The protection effect could be attributed to antioxidant capacity of DPDT at this concentration range in V79 cells. |
| Databáze: | OpenAIRE |
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