Clinical magnetic resonance-enabled characterization of mono-iodoacetate-induced osteoarthritis in a large animal species

Autor: Rahul Kanwar, Mark D. Unger, Timothy P. Maus, Kasey A. Strand, Naveen S. Murthy, Andreas S. Beutler
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Knee Joint
Swine
Knees
Arthritis
lcsh:Medicine
Knee Joints
Osteoarthritis
Pathology and Laboratory Medicine
Injections
Intra-Articular
Diagnostic Radiology
0302 clinical medicine
Edema
Medicine and Health Sciences
Body Size
030212 general & internal medicine
lcsh:Science
Musculoskeletal System
Ultrasonography
Mammals
Multidisciplinary
medicine.diagnostic_test
Radiology and Imaging
Eukaryota
Osteoarthritis
Knee
Magnetic Resonance Imaging
Iodoacetic Acid
3. Good health
medicine.anatomical_structure
Connective Tissue
Vertebrates
Disease Progression
Legs
Anatomy
medicine.symptom
Research Article
musculoskeletal diseases
Imaging Techniques
Research and Analysis Methods
Lesion
03 medical and health sciences
Signs and Symptoms
Rheumatology
Diagnostic Medicine
medicine
Animals
Humans
Grading (tumors)
030203 arthritis & rheumatology
business.industry
Cartilage
Limbs (Anatomy)
lcsh:R
Organisms
Biology and Life Sciences
Magnetic resonance imaging
medicine.disease
Disease Models
Animal
Joints (Anatomy)
Biological Tissue
Amniotes
lcsh:Q
Nuclear medicine
business
Large animal
Zdroj: PLoS ONE, Vol 13, Iss 8, p e0201673 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Introduction Osteoarthritis (OA) is the most common form of arthritis. Medical and surgical treatments have yet to substantially diminish the global health and economic burden of OA. Due to recent advances in clinical imaging, including magnetic resonance imaging (MRI), a correlation has been established between structural joint damage and OA-related pain and disability. Existing preclinical animal models of OA are useful tools but each suffers specific roadblocks when translating structural MRI data to humans. Intraarticular injection of mono-iodoacetate (MIA) is a reliable, well-studied method to induce OA in small animals but joint size discrepancy precludes the use of clinical grade MRI to study structural disease. The porcine knee is suited for clinical MRI and demonstrates homology with humans. We set out to establish the first large animal model of MIA-induced knee OA in swine characterized by structural MRI. Materials and methods Yucatan swine (n = 27) underwent ultrasound-guided injection of knees with 1.2, 4, 12, or 40 mg MIA. MRI was performed at several time points over 12 weeks (n = 54 knees) and images were assessed according to a modified clinical grading scheme. Knees were harvested and graded up to 35 weeks after injection. Results MIA-injected knees (n = 25) but not control knees (n = 29) developed gross degeneration. A total of n = 6,000 MRI measurements were recorded by two radiologists. MRI revealed progressive cartilage damage, bone marrow edema, erosions, and effusions in MIA-injected knees. Lesion severity and progression was influenced by time, dose, and inter-individual variability. Conclusions Intraarticular injection of MIA produced structural knee degradation that was reliably characterized using clinical MRI in swine. Destruction was progressive and, similar to human OA, lesion severity was heterogeneous between and within treatment groups.
Databáze: OpenAIRE
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