Association of Microbial Dysbiosis with Gallbladder Diseases Identified by Bile Microbiome Profiling
| Autor: | Kiseok Jang, Yun Kyung Jung, Yeseul Kim, Ho Soon Choi, Su Jin Shin, Jehyun Jeon, Yohan Kim, Sung-Gyu Lee, Kyeong Geun Lee, Mimi Kim, Seong Ji Choi, Dongho Choi, Mina Rho, Yangsoon Lee, Dong Hwan Jung, Jaemin Jeong, Ho Jin Gwak, Kyojin Kang |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
medicine.medical_specialty Gallbladder disease Gallbladder Diseases medicine.disease_cause Gastroenterology Internal medicine medicine Cholecystitis Bile Humans Microbiome Gallbladder cancer Phylogeny Bacteria Gastroenterology & Hepatology business.industry Gallbladder Microbiota Chronic Cholecystitis General Medicine Gallstones Middle Aged medicine.disease medicine.anatomical_structure Case-Control Studies Dysbiosis Gallbladder Neoplasms Original Article Metagenomics Gallbladder Neoplasm business Carcinogenesis |
| Zdroj: | Journal of Korean Medical Science |
| ISSN: | 1598-6357 |
| Popis: | Background Cholecystitis is an important risk factor for gallbladder cancer, but the bile microbiome and its association with gallbladder disease has not been investigated fully. We aimed to analyze the bile microbiome in normal conditions, chronic cholecystitis, and gallbladder cancer, and to identify candidate bacteria that play an important role in gallbladder carcinogenesis. Methods We performed metagenome sequencing on bile samples of 10 healthy individuals, 10 patients with chronic cholecystitis, and 5 patients with gallbladder cancer, and compared the clinical, radiological, and pathological characteristics of the participants. Results No significant bacterial signal was identified in the normal bile. The predominant dysbiotic bacteria in both chronic cholecystitis and gallbladder cancer were those belonging to the Enterobacteriaceae family. Klebsiella increased significantly in the order of normal, chronic cholecystitis, and gallbladder cancer. Patients with chronic cholecystitis and dysbiotic microbiome patterns had larger gallstones and showed marked epithelial atypia, which are considered as precancerous conditions. Conclusion We investigated the bile microbiome in normal, chronic cholecystitis, and gallbladder cancer. We suggest possible roles of Enterobacteriaceae, including Klebsiella, in gallbladder carcinogenesis. Our findings reveal a possible link between a dysbiotic bile microbiome and the development of chronic calculous cholecystitis and gallbladder cancer. Graphical Abstract |
| Databáze: | OpenAIRE |
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