Investigation of an Outbreak of Dengue Virus Serotype 1 in a Rural Area of Kribi, South Cameroon: A Cross-Sectional Study
| Autor: | Jean Joel Bigna, Fredy Brice N. Simo, Francine Berlange Sado Yousseu, Armel Evouna Mbarga, Armel Melong, Paul Moundipa Fewou, Maurice Demanou, Basile Kamgang, Raphael Bouyne, Anicet Ntoude |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Rural Population Serotype Cross-sectional study viruses 030231 tropical medicine Population Enzyme-Linked Immunosorbent Assay Dengue virus Antibodies Viral Real-Time Polymerase Chain Reaction Serogroup medicine.disease_cause Disease Outbreaks Dengue fever Dengue 03 medical and health sciences 0302 clinical medicine Virology Pandemic medicine Humans Cameroon 030212 general & internal medicine Chikungunya education education.field_of_study virus diseases Outbreak Dengue Virus medicine.disease Cross-Sectional Studies Infectious Diseases Geography Research Design Female |
| Zdroj: | Intervirology. 61:265-271 |
| ISSN: | 1423-0100 0300-5526 |
| Popis: | Background: On May 2017, a case of dengue serotype 1 was detected and confirmed through routine surveillance in a traveler returning from Kribi, a seaside town of Southern Cameroon. This study aimed at confirming the circulation of dengue virus (DENV) in Southern Cameroon. Methods: A cross sectional study was carried out in Londji near Kribi from June 21–25, 2017, by a joint team of Centre Pasteur of Cameroon and the Department of Diseases, Epidemics and Pandemics Control. Blood samples of consented participants were collected and tested for anti-D ENV IgM using an IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA), and for the detection of Zika, dengue, or chikungunya viruses using Trioplex real-time reverse transcription-polymerase chain reaction (RT-PCR). DENV RNA-positive samples were serotyped using an end-point nested RT-PCR. Results: Ninety-one participants were enrolled, 50.55% (46/91) of them males. The mean age of the population was 30.71 years (±18.89). In total, 14.28% (13/91) of the participants had DENV infection (3 anti-DENV IgM positive and 10 DENV serotype 1 RT-PCR positive). Conclusion: The detection of DENV serotype 1 in an autochthonous population during this survey is a confirmation that the seaside city of Kribi is a risk area for contracting dengue infection in Cameroon. |
| Databáze: | OpenAIRE |
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