Immunolocalization of Cancer Stem Cells Marker ALDH1 and its Association with Tumor Budding in Oral Squamous Cell Carcinoma
| Autor: | Martinho Campolina Rebello Horta, Ângela Braga Caixeta, Helvécio Marangon Junior, Maria Cássia Ferreira de Aguiar, Giovanna Ribeiro Souto, Paulo Eduardo Alencar Souza, Victória Vasconcellos Moreira Melo |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Biology medicine.disease_cause Aldehyde Dehydrogenase 1 Family Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Tumor budding Cancer stem cell Biomarkers Tumor medicine Humans Basal cell Treatment resistance Aged Original Paper Budding Squamous Cell Carcinoma of Head and Neck Middle Aged Immunohistochemistry Phenotype stomatognathic diseases 030104 developmental biology Oncology Otorhinolaryngology 030220 oncology & carcinogenesis Neoplastic Stem Cells Female Mouth Neoplasms Carcinogenesis |
| Zdroj: | Head and Neck Pathology. 13:535-542 |
| ISSN: | 1936-0568 |
| DOI: | 10.1007/s12105-018-0985-4 |
| Popis: | Tumor budding is a prognostic marker for oral squamous cell carcinoma (OSCC) characterized by the presence of isolated or small clusters of neoplastic cells at the tumor invasive front. Aldehyde dehydrogenase-1 (ALDH1) is associated with tumorigenesis, linked to treatment resistance and shown to identify cancer stem cells (CSC)-like cells. This study aimed to evaluate the expression of ALDH1 and its association with tumor budding in OSCC. Immunohistochemistry was employed in 163 OSCC samples to identify pancytokeratin (AE1/AE3) and ALDH1. While pancytokeratin (AE1/AE3) identified squamous tumor buds, the CSC-like cells were identified using ALDH1. A Chi square test was used to evaluate association between ALDH1 expression and tumor budding, while McNemar’s test was used to identify differences in ALDH1 expression between the budding area and the area outside the budding. A positive expression of ALDH1 was observed in 47.24% of the samples and in 70% of anatomic locations affected. No association was observed between ALDH1 expression and tumor budding (p > 0.05). In tumors with high-intensity tumor budding, ALDH1 expression was higher in the budding area than in the area outside the budding (p |
| Databáze: | OpenAIRE |
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