Rapid typing of transmissible spongiform encephalopathy strains with differential ELISA
| Autor: | Muriel Feyssaguet, Nathalie Morel, Jean-Marc Bilheude, Hervé Boutal, Jacques Grassi, Jérôme Nugier, Christophe Créminon, Stéphanie Simon, Olivier Andreoletti, Sylvie L. Benestad, Frédéric Lantier, Anne-Gaëlle Biacabe, Thierry Baron |
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| Přispěvatelé: | Commissariat à l'Energie Atomique, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), National Veterinary Institute, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Bio-Rad, BIO-RAD, Laboratoire d'études et de recherches en pathologie bovine et hygiène des viandes, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Infection Animale et Santé Publique, AFSSA |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Epidemiology
animal diseases lcsh:Medicine Scrapie BSE 0403 veterinary science [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases MESH: Animals 0303 health sciences Goat Diseases Transmissible spongiform encephalopathy biology Goats food and beverages MESH: Enzyme-Linked Immunosorbent Assay 04 agricultural and veterinary sciences XUATNC HAFSSA Animals Cattle Encephalopathy Bovine Spongiform Enzyme-Linked Immunosorbent Assay Prions Sheep 3. Good health Encephalopathy Bovine Spongiform MESH: Cattle Infectious Diseases MESH: Scrapie Veterinary medicine and animal Health ELISA SDV:MHEP:MI Microbiology (medical) MESH: Encephalopathy Bovine Spongiform 040301 veterinary sciences Bovine spongiform encephalopathy MESH: Sheep Context (language use) MESH: Goat Diseases MESH: Goats lcsh:Infectious and parasitic diseases 03 medical and health sciences MESH: Prions mental disorders medicine lcsh:RC109-216 Typing Prion protein 030304 developmental biology PrP [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health Research lcsh:R scrapie Proteinase K medicine.disease Virology nervous system diseases Médecine vétérinaire et santé animal nervous system biology.protein strain typing |
| Zdroj: | Emerging Infectious Diseases Emerging Infectious Diseases, 2008, 14 (4), pp.608-16 Emerging Infectious Diseases, Centers for Disease Control and Prevention, 2008, 14 (4), pp.608-16 HAL Emerging Infectious Diseases, Vol 14, Iss 4, Pp 608-616 (2008) Emerging Infectious Diseases 4 (14), 608-16. (2008) |
| ISSN: | 1080-6040 1080-6059 |
| Popis: | A strain-typing ELISA distinguishes bovine spongiform encephalopathy from other scrapie strains in small ruminants. The bovine spongiform encephalopathy (BSE) agent has been transmitted to humans, leading to variant Creutzfeldt-Jakob disease. Sheep and goats can be experimentally infected by BSE and have been potentially exposed to natural BSE; however, whether BSE can be transmitted to small ruminants is not known. Based on the particular biochemical properties of the abnormal prion protein (PrPsc) associated with BSE, and particularly the increased degradation induced by proteinase K in the N terminal part of PrPsc, we have developed a rapid ELISA designed to distinguish BSE from other scrapie strains. This assay clearly discriminates experimental ovine BSE from other scrapie strains and was used to screen 260 transmissible spongiform encephalopathy (TSE)–infected small ruminant samples identified by the French active surveillance network (2002/2003). In this context, this test has helped to identify the first case of natural BSE in a goat and can be used to classify TSE isolates based on the proteinase K sensitivity of PrPsc. |
| Databáze: | OpenAIRE |
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