A family of transmembrane microneme proteins of Toxoplasma gondii contain EGF-like domains and function as escorters

Autor: Matthias Reiss, Nicola K. Viebig, James W. Ajioka, Markus Meissner, Dominique Soldati, Catherine Toursel, Vern B. Carruthers, Stanislas Tomavo
Rok vydání: 2002
Předmět:
Protein family
Recombinant Fusion Proteins
Molecular Sequence Data
Protozoan Proteins
Protein Sorting Signals
Recombinant Fusion Proteins/genetics/metabolism
Microneme
Protein Transport/physiology
Animals
Humans
Secretion
Epidermal Growth Factor/chemistry/genetics
Amino Acid Sequence
Protozoan Proteins/chemistry/genetics/metabolism
Adhesins
Bacterial
Secretory pathway
ddc:616
biology
Epidermal Growth Factor
Carrier Proteins/chemistry/genetics/metabolism
Toxoplasma gondii
Gene Expression Regulation
Developmental
Membrane Proteins
Cell Biology
biology.organism_classification
Transmembrane protein
Cell biology
Protein Structure
Tertiary
Protein Transport
Cytoplasm
Membrane topology
Membrane Proteins/chemistry/genetics/metabolism
Immunology
Carrier Proteins
Sequence Alignment
Toxoplasma
Toxoplasma/cytology/physiology
Zdroj: Journal of Cell Science, Vol. 115, No Pt 3 (2002) pp. 563-74
Scopus-Elsevier
ISSN: 0021-9533
Popis: TgMIC6, TgMIC7, TgMIC8 and TgMIC9 are members of a novel family of transmembrane proteins localized in the micronemes of the protozoan parasite Toxoplasma gondii. These proteins contain multiple epidermal growth factor-like domains, a putative transmembrane spanning domain and a short cytoplasmic tail. Sorting signals to the micronemes are encoded in this short tail. We established previously that TgMIC6 serves as an escorter for two soluble adhesins, TgMIC1 and TgMIC4. Here, we present the characterization of TgMIC6 and three additional members of this family, TgMIC7, -8 and -9. Consistent with having sorting signals localized in its C-terminal tail,TgMIC6 exhibits a classical type I membrane topology during its transport along the secretory pathway and during storage in the micronemes. TgMIC6 is processed at the N-terminus, probably in the trans-Golgi network, and the cleavage site has been precisely mapped. Additionally, like other members of the thrombospondin-related anonymous protein family, TgMIC2, TgMIC6 and TgMIC8 are proteolytically cleaved near their C-terminal domain upon discharge by micronemes. We also provide evidence that TgMIC8 escorts another recently described soluble adhesin, TgMIC3. This suggests that the existence of microneme protein complexes is not an exception but rather the rule. TgMIC6 and TgMIC8 are expressed in the rapidly dividing tachyzoites, while TgMIC7 and TgMIC9 genes are predominantly expressed in bradyzoites, where they presumably also serve as escorters.
Databáze: OpenAIRE
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