Translational sensitivity of the Escherichia coli genome to fluctuating tRNA availability
| Autor: | Johannes Andries Roubos, Sibylle E. Wohlgemuth, Thomas E. Gorochowski |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Biology
Genome Ribosome 03 medical and health sciences 0302 clinical medicine RNA Transfer Escherichia coli Genetics Protein biosynthesis Codon Gene 030304 developmental biology 0303 health sciences Bristol BioDesign Institute Computational Biology RNA Translation (biology) Protein Biosynthesis Transfer RNA Translational elongation 5' Untranslated Regions Genome Bacterial 030217 neurology & neurosurgery |
| Zdroj: | Nucleic Acids Research Wohlgemuth, S E, Gorochowski, T E & Roubos, J A 2013, ' Translational sensitivity of the Escherichia coli genome to fluctuating tRNA availability ', Nucleic Acids Research, vol. 41, no. 17, pp. 8021-33 . https://doi.org/10.1093/nar/gkt602 |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | The synthesis of protein from messenger RNA during translation is a highly dynamic process that plays a key role in controlling the efficiency and fidelity of genome-wide protein expression. The availability of aminoacylated transfer RNA (tRNA) is a major factor influencing the speed of ribosomal movement, which depending on codon choices, varies considerably along a transcript. Furthermore, it has been shown experimentally that tRNA availability can vary significantly under different growth and stress conditions, offering the cell a way to adapt translational dynamics across the genome. Existing models of translation have neglected fluctuations of tRNA pools, instead assuming fixed tRNA availabilities over time. This has lead to an incomplete understanding of this process. Here, we show for the entire Escherichia coli genome how and to what extent translational speed profiles, which capture local aspects of translational elongation, respond to measured shifts in tRNA availability. We find that translational profiles across the genome are affected to differing degrees, with genes that are essential or related to fundamental processes such as translation, being more robust than those linked to regulation. Furthermore, we reveal how fluctuating tRNA availability influences profiles of specific sequences known to play a significant role in translational control of gene expression. |
| Databáze: | OpenAIRE |
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