A novel type I interferon, interferon alphaomega, shows antiviral activity against foot-and-mouth disease virus in vitro
| Autor: | Shi-fang Li, Huiyun Chang, Fu-rong Zhao, Yin-li Xie, Yong-guang Zhang, Jun–jun Shao, Gong Meijiao |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Swine 030106 microbiology Antiviral Agents Microbiology Cell Line 03 medical and health sciences Cytopathogenic Effect Viral Interferon medicine Animals Immunologic Factors Gene Cytopathic effect biology Gene Expression Profiling Biological activity biology.organism_classification Virology ISG15 Protein kinase R Recombinant Proteins In vitro 030104 developmental biology Infectious Diseases Foot-and-Mouth Disease Virus Interferon Type I Foot-and-mouth disease virus medicine.drug |
| Zdroj: | Microbial Pathogenesis. 127:79-84 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2018.11.040 |
| Popis: | Recently, a novel type I interferon alphaomega (IFN-αω), also known as IFN-μ, was identified. However, the biological activity of IFN-αω remain poorly understood. In this study, the porcine IFN-αω (PoIFN-αω) was expressed, purified, and its antiviral activities assessed by its ability to inhibit the cytopathic effect caused by FMDV on IBRS-2 cells. In addition, q-PCR was used to evaluate the expression of IFN-stimulated genes induced by PoIFN-αω. It was found that PoIFN-αω exerted effective antiviral activity against FMDV pre- and post-infection. Additionally, PoIFN-αω induced the transcription of IFN-stimulated genes, including Mx1, ISG15, OAS1, and PKR genes. Our study reported a new indication of PoIFN-αω as an effective anti-FMDV agent for the first time. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect