Ultrasound molecular imaging of arterial thrombi with novel microbubbles modified by cyclic RGD in vitro and in vivo
| Autor: | Cheng Liu, Ruizhu Huang, Kaviraj Bundhoo, Guang-quan Hu, Jianping Bin, Jiajia Xie, Juefei Wu, Ying Liu, Lic Yang, Yulin Liao |
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| Rok vydání: | 2012 |
| Předmět: |
Blood Platelets
medicine.medical_specialty Time Factors Myocytes Smooth Muscle Peptide 02 engineering and technology 010402 general chemistry 01 natural sciences Muscle Smooth Vascular In vivo medicine Animals Humans Ultrasonics Rats Wistar Thrombus Receptor chemistry.chemical_classification Microbubbles Chemistry Echogenicity Thrombosis Arteries Hematology 021001 nanoscience & nanotechnology medicine.disease In vitro Rats 0104 chemical sciences cardiovascular system Stress Mechanical Radiology Peptides Shear Strength 0210 nano-technology Molecular probe Oligopeptides Biomedical engineering |
| Zdroj: | Thrombosis and Haemostasis. 107:172-183 |
| ISSN: | 2567-689X 0340-6245 |
| DOI: | 10.1160/th10-11-0701 |
| Popis: | SummaryDespite immense potential, ultrasound molecular imaging (UMI) of arterial thrombi remains very challenging because the high-shear arterial flow limits binding of site-targeted microbubbles to the thrombi. The linear Arg-Gly-Asp (RGD) peptides have been successfully applied to evaluate venous, atrial, and arteriolar thrombi, but have thus far failed in the detection of arterial thrombi. Cyclic RGD (Arg-Gly-Asp-D-Phe-Cys) is a cyclic conformation of linear RGD peptides, which has much higher binding-affinity and selectivity for binding to the glycoprotein (GP) IIb/IIIa receptor than its linear counterpart and thus is likely to be an optimal targeted molecular probe for ultrasound molecular imaging of arterial thrombi. In this study, we sought to assess the feasibility of a novel microbubble conjugated with cyclic RGD (Mb-cyclic RGD) in UMI of arterial thrombi in vitro and in vivo. As expected, Mb-cyclic RGD had greater GP IIb/IIIa-targeted binding capability in all shear stress conditions. In addition, the shear stress at half-maximal detachment of Mb-cyclic RGD was 5.7-fold higher than that of microbubbles with nonspecific peptide (Mb-CON) (p |
| Databáze: | OpenAIRE |
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