Dengue-4 vaccine: neurovirulence, viraemia and immune responses in rhesus and cynomolgus monkeys
| Autor: | Somphong Sahaphong, S. Angsubhakorn, Sutee Yoksan, Natth Bhamarapravati, Nyven J. Marchette, James B. Moe, Apichat Pradermwong |
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| Rok vydání: | 1988 |
| Předmět: |
viruses
Viremia Antibodies Viral Vaccines Attenuated Virus Dengue fever Dengue Immune system Neutralization Tests medicine Animals Potency Neutralizing antibody Brain Diseases Kidney Virulence biology Immunogenicity Public Health Environmental and Occupational Health Viral Vaccines General Medicine Dengue Virus Hemagglutination Inhibition Tests medicine.disease Macaca mulatta Virology Macaca fascicularis Infectious Diseases medicine.anatomical_structure Immunology biology.protein Parasitology |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 82:746-749 |
| ISSN: | 0035-9203 |
| DOI: | 10.1016/0035-9203(88)90224-6 |
| Popis: | A dengue 4 (DEN-4, strain 1036-PDK 48) vaccine attenuated by passage in primary dog kidney cells was tested using rhesus (Macaca mulatta) and cynomolgus (M. fascicularis) monkeys to determine its safety, potency, and immunogenicity. 14 rhesus monkeys were divided into 3 groups: group 1, 2 animals given control culture fluid; group 2, 2 animals given DEN-4 parental virus; group 3, 10 animals given DEN-4 vaccine virus. 10 cynomolgus were similarly grouped, but group 3 contained 6 monkeys. No significant neurovirulence was observed with parental or with DEN-4 virus passaged in primary dog kidney (PDK) cells. Both cynomolgus monkeys inoculated with DEN-4 vaccine virus developed minimal (V-1) and mild (V-2) neurovirulence-type lesions in the central nervous system, which were nondestructive in both species. All parental and vaccine viruses produced moderate to high neutralizing antibody titres. Only parental virus produced viraemia, in 2 cynomolgus monkeys. Because of its safety and avirulence in monkeys, PDK 48 is recommended for human trial. |
| Databáze: | OpenAIRE |
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