Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics
| Autor: | Jiang Tao, Joseph M. Kinsella, Joel Grant, Sanahan Vijayakumar, Allen J. Ehrlicher, Antonio De León-Rodríguez, Jacqueline Kort-Mascort, Jose G. Munguia-Lopez, Salvador Flores-Torres, Kevin Gu, Maeva M Bavoux |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
food.ingredient
Alginates 0206 medical engineering Composite number Biomedical Engineering Bioengineering Breast Neoplasms 02 engineering and technology Biochemistry Gelatin Biomaterials food In vivo Cell Line Tumor Spheroids Cellular Cell Adhesion Humans Cell adhesion Cell Proliferation Tissue Engineering Tissue Scaffolds Chemistry Spheroid Bioprinting Hydrogels General Medicine 021001 nanoscience & nanotechnology 020601 biomedical engineering In vitro Kinetics Printing Three-Dimensional Biophysics Adhesive 0210 nano-technology Biotechnology Biofabrication |
| Zdroj: | Biofabrication. 12(1) |
| ISSN: | 1758-5090 |
| Popis: | Tunable bioprinting materials are capable of creating a broad spectrum of physiological mimicking 3D models enabling in vitro studies that more accurately resemble in vivo conditions. Tailoring the material properties of the bioink such that it achieves both bioprintability and biomimicry remains a key challenge. Here we report the development of engineered composite hydrogels consisting of gelatin and alginate components. The composite gels are demonstrated as a cell-laden bioink to build 3D bioprinted in vitro breast tumor models. The initial mechanical characteristics of each composite hydrogel are correlated to cell proliferation rates and cell spheroid morphology spanning month long culture conditions. MDA-MB-231 breast cancer cells show gel formulation-dependency on the rates and frequency of self-assembly into multicellular tumor spheroids (MCTS). Hydrogel compositions comprised of decreasing alginate concentrations, and increasing gelatin concentrations, result in gels that are mechanically soft and contain a greater number of cell-adhesion moieties driving the development of large MCTS; conversely gels containing increasing alginate, and decreasing gelatin concentrations are mechanically stiffer, with fewer cell-adhesion moieties present in the composite gels yielding smaller and less viable MCTS. These composite hydrogels can be used in the biofabrication of tunable in vitro systems that mimic both the mechanical and biochemical properties of the native tumor stroma. |
| Databáze: | OpenAIRE |
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