A Manual Spectrophotometric Method for the Measurement of Serum Sodium and Potassium by Enzyme Activation
| Autor: | B C Mazzachi, M N Berry, R D Mazzachi |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Sodium
Potassium education Clinical Biochemistry chemistry.chemical_element Electrolyte Absorption Enzyme activator Spectrophotometry medicine Humans Drug Interactions Edetic Acid Triglycerides Chromatography medicine.diagnostic_test Biochemistry (medical) Pipette Reproducibility of Results Sodium Dodecyl Sulfate Bilirubin General Medicine Reference Standards Enzyme Activation Biochemistry chemistry Reagent Calibration Linear Models Spectrophotometry Ultraviolet Quantitative analysis (chemistry) Mathematics |
| Zdroj: | cclm. 32:709-718 |
| ISSN: | 1437-4331 1434-6621 |
| DOI: | 10.1515/cclm.1994.32.9.709 |
| Popis: | Manual procedures suitable for use on standard benchtop spectrophotometers have been developed for the enzymatic determination of Na+ and K+ in serum. Both assays require only minimal modification of reagents already available for BM/Hitachi analyzers and are performed in an endpoint mode, allowing up to 20 assays per run. The addition of a stop reagent is required--dipotassium EDTA for the Na+ assay and sodium dodecyl sulphate for the K+ assay. The most important criterion for achieving good assay performance is the precise pipetting of sample and reagent. Within-run imprecision is < 1% for Na+ and K+, and between-run imprecision < 1.5%, for both assays at all but the lowest concentrations of K+. Enzymatic electrolyte results compare well with flame photometry, however the assays are more prone to interference by very high concentrations of bilirubin or triacylglycerols than those performed on automated, dual-wavelength kinetic analyzers. It is possible to correct for most interferences by inclusion of appropriate sample and reagent blanks. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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