Detection of dengue nonstructural 1 (NS1) protein in Vietnamese patients with fever
| Autor: | Nguyen V. Nam, Tran Thi Thanh Nga, Le Q. Hung, Khoa T. D. Thai, Jan Groen, Hoang Lan Phuong, Tran Quang Binh, Peter J. de Vries, Phan T. Giao |
|---|---|
| Přispěvatelé: | Infectious diseases |
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Microbiology (medical) medicine.medical_specialty Adolescent Statistics as Topic Enzyme-Linked Immunosorbent Assay Viral Nonstructural Proteins Antibodies Viral Fever of Unknown Origin law.invention Dengue fever Dengue Young Adult Predictive Value of Tests law Internal medicine Positive predicative value Primary health medicine Humans Child Polymerase chain reaction biology Reverse Transcriptase Polymerase Chain Reaction General Medicine Elisa assay Dengue Virus medicine.disease Virology Infectious Diseases Vietnam biology.protein RNA Viral Female Viral disease Antibody Malaria |
| Zdroj: | Diagnostic microbiology and infectious disease, 63(4), 372-378. Elsevier Inc. |
| ISSN: | 0732-8893 |
| Popis: | Diagnostic dengue in febrile patients is challenging. Of a total of 459 patients with acute undifferentiated fever, randomly selected from 12 primary health facilities and 1 clinic of the provincial malaria station in southern Vietnam, dengue-specific antibody (Ab) and NS1 Ag enzyme-linked immunosorbent assay (ELISA) (Platelia (TM) Bio-Rad Laboratories, Hercules, Ca 94547. US) were performed on acute (t3) and convalescent (t3 weeks) sera. Polymerase chain reaction (PCR) was used for confirmation. Based on a composite of the NS1 Ag-ELISA, Ab-ELISA, and PCR results. 54 (12%) patients had acute dengue. Positive and negative predictive values were 65% and 98% for the Ab-based diagnosis and 91% and 92% for NS1Ag, respectively. The agreement between Ab- and NS1Ag-based diagnosis was poor (k value, 0.2). Two patients without dengue had detectable NS1Ag on t0 and t3.1 just above the cutoff value and 1 with very high values. For 5 dengue patients, NS1Ag was still detectable at very high levels at t3. Dengue NS1Ag can be used for early diagnosis of dengue; infrequent false-positive results need further clarification. (C) 2009 Elsevier Inc. All rights reserved |
| Databáze: | OpenAIRE |
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