Enhancing the biological performance of osteoconductive nanocomposite scaffolds through negative voltage electrospinning
| Autor: | William W. Lu, Ho-Wang Tong, Min Wang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Scaffold Nanocomposite Materials science Tissue Engineering Tissue Scaffolds Polymers Composite number Biomedical Engineering Medicine (miscellaneous) Nanoparticle Bioengineering Polymer Development Matrix (biology) Electrospinning Nanocomposites Durapatite chemistry Chemical engineering Spectroscopy Fourier Transform Infrared General Materials Science Lactic Acid Fiber Composite material |
| Zdroj: | Nanomedicine. 8:577-589 |
| ISSN: | 1748-6963 1743-5889 |
| Popis: | Aim: To investigate negative voltage electrospinning of fibrous nanocomposite scaffolds bearing negative electric charges (N-poled), and determine whether and how retained negative charges could influence the biological performance of scaffolds for bone tissue engineering. Materials & methods: Poly(D,L-lactic acid) was used as the polymer matrix and carbonated hydroxyapatite nanospheres were the osteoconductive phase in the electrospun nanocomposite scaffolds. N-poled nanocomposite scaffolds were formed using negative voltage electrospinning, while conventional positive voltage electrospinning produced fibrous nanocomposite scaffolds bearing positive electric charges (P-poled). N-poled and P-poled scaffolds were systematically characterized and their biological performance was investigated through in vitro cell culture experiments. Results & conclusion: N-poled and P-poled scaffolds retained charges for different periods of time after electrospinning. Both types of scaffolds supported cell spreading and promoted filopodia formation. Compared with P-poled scaffolds, N-poled scaffolds enhanced cell proliferation, alkaline phosphatase activity and mineralization. N-poled scaffolds offer distinct advantages for bone tissue engineering. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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