Detection of Anaplasma phagocytophilum in Wild and Farmed Cervids in Poland
| Autor: | Zdzisław Laskowski, Żaneta Steiner-Bogdaszewska, Michał Czopowicz, Anna W. Myczka, Katarzyna Filip-Hutsch, Grzegorz Oloś |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
16S rDNA animal diseases Zoology Spleen Article Anaplasma phagocytophilum Liver tissue biology.animal parasitic diseases medicine Immunology and Allergy Natural reservoir Anaplasma Molecular Biology General Immunology and Microbiology biology fungi biology.organism_classification 16S ribosomal RNA bacterial infections and mycoses Roe deer Infectious Diseases medicine.anatomical_structure wild cervids Medicine bacteria Nested polymerase chain reaction farm animals |
| Zdroj: | Pathogens Volume 10 Issue 9 Pathogens, Vol 10, Iss 1190, p 1190 (2021) |
| ISSN: | 2076-0817 |
| Popis: | Background: The role of cervids in the circulation of A. phagocytophilum has not yet been clearly determined however, several species of wild and farm cervids may be a natural reservoir of this bacteria. Methods: Spleen and liver tissue samples were taken from 207 wild (red deer, roe deer, fallow deer and moose) and farmed cervids (red deer and fallow deer) from five geographical areas. These were tested for the A. phagocytophilum16S rDNA partial gene by nested PCR. Results: Anaplasma spp. were detected in 91 of 207 examined cervids (prevalence 43.9%). Three different variants of 16S rDNA partial gene were reported, one for the first time. Anaplasma phagocytophilum was more often detected in young specimens than in adults and more often in the spleen than in the liver. Conclusions: Cervids from the four sites across Poland were found to be major natural reservoirs of various strains of A. phagocytophilum. This is the first study to use spleen and liver as biological material to detect A. phagocytophilum in moose in Poland. |
| Databáze: | OpenAIRE |
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