Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor
| Autor: | Kazuko Yamaguchi-Shinozaki, Qiang Liu, Setsuko Miura, Kazuo Shinozaki, Mie Kasuga |
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| Rok vydání: | 1999 |
| Předmět: |
Time Factors
Response element Drought tolerance Arabidopsis Biomedical Engineering Gene Expression Bioengineering Genetically modified crops Sodium Chloride Applied Microbiology and Biotechnology Disasters Gene expression Arabidopsis thaliana Promoter Regions Genetic Gene Transcription factor Genetics biology Arabidopsis Proteins fungi Temperature food and beverages Blotting Northern Plants Genetically Modified biology.organism_classification Cell biology Phenotype Molecular Medicine Cauliflower mosaic virus Transcription Factors Biotechnology |
| Zdroj: | Novartis Foundation Symposium 236-Rice Biotechnology: Improving Yield, Stress Tolerance and Grain Quality |
| ISSN: | 1546-1696 1087-0156 |
| DOI: | 10.1038/7036 |
| Popis: | Plant productivity is greatly affected by environmental stresses such as drought, salt loading, and freezing. We reported previously that a cis-acting promoter element, the dehydration response element (DRE), plays an important role in regulating gene expression in response to these stresses. The transcription factor DREB1A specifically interacts with the DRE and induces expression of stress tolerance genes. We show here that overexpression of the cDNA encoding DREB1A in transgenic plants activated the expression of many of these stress tolerance genes under normal growing conditions and resulted in improved tolerance to drought, salt loading, and freezing. However, use of the strong constitutive 35S cauliflower mosaic virus (CaMV) promoter to drive expression of DREB1A also resulted in severe growth retardation under normal growing conditions. In contrast, expression of DREB1A from the stress inducible rd29A promoter gave rise to minimal effects on plant growth while providing an even greater tolerance to stress conditions than did expression of the gene from the CaMV promoter. |
| Databáze: | OpenAIRE |
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