| Popis: |
A study was made of the effect of various conditions on the stability of mittochondria isolated from rat liver. The pH of the solution was found to be much more important than the concentration of sucrose in maintaining the stability of these organelles. ATP, ADP, Mg, Ca, versene, and DNP were found to prevent the swelling of isolated mitochondria in hypotonic sucrose at pH 8.0. Calcium was found to have a slight destructive action under hypertonic conditions. The destructive action of phosphate was found to be dependent on hypotonic conditions whereas that of succinate could be observed only in hypertonic suspensions. Although the mitochondria became markedly more unstable to alkaline conditions after short periods of aging at 37° the protective mechanisms utilizing ATP and magnesium are lost more slowly. Little difference in stability was noted among preparations of mitochondria which were round and swollen, “rod” shaped, or well preserved morphologically. Accordingly the alteration in morphology during the process of isolation of the mitochondria probably did not influence the stability of these organelles in the present experiments or in those of others. |
