Engineering Staphylococcal Protein A for high-throughput affinity purification of monoclonal antibodies
| Autor: | Vinod Amritkar, Satish Adat, Anurag S. Rathore, Vijay Tejwani, Rahul Bhambure |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
medicine.drug_class Bioengineering Monoclonal antibody Ligands Protein Engineering 01 natural sciences Applied Microbiology and Biotechnology Chromatography Affinity law.invention 03 medical and health sciences Affinity chromatography law 010608 biotechnology medicine Staphylococcal Protein A 030304 developmental biology 0303 health sciences biology Chemistry Elution Antibodies Monoclonal Protein engineering Ligand (biochemistry) Biopharmaceutical Biochemistry Recombinant DNA biology.protein Protein A Biotechnology |
| Zdroj: | Biotechnology advances. 44 |
| ISSN: | 1873-1899 |
| Popis: | Protein A chromatography is one of the most widely used purification steps in the manufacturing of the various classes of recombinant and non-recombinant antibodies. Due to the higher cost, lower binding capacity, and limited life cycle of Protein A ligand, this affinity-based purification step is often one of the most significant contributors to the cost of manufacturing of monoclonal antibody (mAb) products. In the last decade, there has been significant progress in improving the Protein A chromatography throughput by designing new engineered Staphylococcal Protein A (SPA) variants with higher dynamic binding capacity, considerable alkaline tolerance, and mild acidic elution pH. This review aims at summarizing the various protein engineering approaches used for improving the throughput of the Protein A-based affinity purification of various immunoglobulins. With biopharmaceutical producers operating under ever-increasing pressure towards reducing the cost of manufacturing, these advances in engineered protein A variants will help in processing larger cell culture volumes with high throughput and thereby significantly lower the cost of raw materials. |
| Databáze: | OpenAIRE |
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