Vaccine Inoculation Route Modulates Early Immunity and Consequently Antigen-Specific Immune Response
| Autor: | Antonio Cosma, Roger Le Grand, Candie Joly, Lev Stimmer, Sébastien Langlois, Nicolas Tchitchek, Frédéric Martinon, André Rodriguez Pozo, Nathalie Dereuddre-Bosquet, Yves Levy, Hakim Hocini, Pierre Rosenbaum, Leslie Gosse, Anne-Sophie Beignon, David Pejoski |
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| Přispěvatelé: | Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes (IMVA-HB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Thérapie génique, Génomique et Epigénomique (U 1169), Service MIRCEN (MIRCEN), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), BEIGNON, Anne-Sophie, Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie François JACOB (JACOB), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Immunologie - Immunopathologie - Immunothérapie [CHU Pitié Salpêtrière] (I3), CHU Charles Foix [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), U1184 IMVA-HB/IDMIT, Immunologie des Maladies Virales et Autoimmunes (IMVA - U1184), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Infectious Diseases Models for Innovative Therapies (IDMIT), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Vaccine Research Institute [Créteil, France] (VRI), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Henri Mondor, Hôpital Albert Chenevier |
| Rok vydání: | 2020 |
| Předmět: |
Male
Injections Intradermal innate & adaptive immune response Immunology Vaccinia virus CD8-Positive T-Lymphocytes Antibodies Viral Vaccines Attenuated Injections Intramuscular 03 medical and health sciences Route of administration chemistry.chemical_compound [SDV.IMM.VAC] Life Sciences [q-bio]/Immunology/Vaccinology 0302 clinical medicine Immune system mass cytometry (CyTOF) Immunity vaccine Vaccinia Animals Medicine 030212 general & internal medicine Original Research 030304 developmental biology 0303 health sciences non human primates Innate immune system business.industry Myeloid-Derived Suppressor Cells Vaccination Viral Vaccines Acquired immune system Antibodies Neutralizing administration routes 3. Good health Macaca fascicularis modified vaccinia Ankara (MVA) chemistry Immunization [SDV.IMM]Life Sciences [q-bio]/Immunology [SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology business |
| Zdroj: | Frontiers in Immunology Frontiers in Immunology, Frontiers, 2021, 12, pp.645210. ⟨10.3389/fimmu.2021.645210⟩ Frontiers in Immunology, 2021, 12, pp.645210. ⟨10.3389/fimmu.2021.645210⟩ Frontiers in Immunology, 2021, 12, ⟨10.3389/fimmu.2021.645210⟩ |
| ISSN: | 1556-5068 1664-3224 |
| Popis: | International audience; Vaccination is one of the most efficient public healthcare measures to fight infectious diseases. Nevertheless, the immune mechanisms induced in vivo by vaccination are still unclear. The route of administration, an important vaccination parameter, can substantially modify the quality of the response. How the route of administration affects the generation and profile of immune responses is of major interest. Here, we aimed to extensively characterize the profiles of the innate and adaptive response to vaccination induced after intradermal, subcutaneous, or intramuscular administration with a modified vaccinia virus Ankara model vaccine in non-human primates. The adaptive response following subcutaneous immunization was clearly different from that following intradermal or intramuscular immunization. The subcutaneous route induced a higher level of neutralizing antibodies than the intradermal and intramuscular vaccination routes. In contrast, polyfunctional CD8 + T-cell responses were preferentially induced after intradermal or intramuscular injection. We observed the same dichotomy when analyzing the early molecular and cellular immune events, highlighting the recruitment of cell populations, such as CD8 + T lymphocytes and myeloid-derived suppressive cells, and the activation of key immunomodulatory gene pathways. These results demonstrate that the quality of the vaccine response induced by an attenuated vaccine is shaped by early and subtle modifications of the innate immune response. In this immunization context, the route of administration must be tailored to the desired type of protective immune response. This will be achieved through systems vaccinology and mathematical modeling, which will be critical for predicting the efficacy of the vaccination route for personalized medicine. |
| Databáze: | OpenAIRE |
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