Lactoperoxidase catalyzes in vitro activation of acrylonitrile to cyanide
| Autor: | Asser I. Ghoneim, Sherry N. Nasralla, Mohamed Z. Gad, Amani E. Khalifa, Ashraf B. Abdel-Naim |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cyanide
Indomethacin Toxicology Medicinal chemistry Catalysis chemistry.chemical_compound Organic chemistry Lactoperoxidase Sulfhydryl Compounds Nitrite Chromans Enzyme Inhibitors Hydrogen peroxide Sodium Azide Biotransformation Cyanides Acrylonitrile Temperature General Medicine Glutathione Free Radical Scavengers Hydrogen Peroxide Resorcinols Hydrogen-Ion Concentration Kinetics chemistry Data Interpretation Statistical Carcinogens Sodium azide Quercetin Trolox |
| Zdroj: | Toxicology letters. 191(2-3) |
| ISSN: | 1879-3169 |
| Popis: | Acrylonitrile (ACN) is a widely used industrial chemical. Although it is a well reported animal carcinogen, its current designation to humans is "possibly carcinogenic". The present study aimed at investigating the ability of LPO enzyme system to oxidize ACN to cyanide (CN(-)) in vitro. Detection of CN(-) served as a marker for the possible generation of free radical intermediates implicated in ACN induced toxicity in the activation process. Optimum conditions for the oxidation of ACN to CN(-) were characterized with respect to pH, temperature and time of incubation as well as ACN, LPO and H(2)O(2) concentrations in incubation mixtures. Maximum reaction velocity (V(max)) and Michaelis-Menten constant (K(m)) were assessed. Addition of nitrite (NO(2)(-)) salts to the reaction mixtures significantly enhanced the rate of the reaction. Free radical scavengers (quercetin and trolox C), LPO enzyme inhibitor (resorcinol) and competitors for LPO binding (sodium azide and indomethacin) were found to reduce the rate of CN(-) production. Inclusion of the sulfhydryl compounds glutathione (GSH), NAC (N-acetylcysteine), D-penicillamine or L-cysteine enhanced the rate of ACN oxidation. The present results demonstrate the ability of LPO enzyme system to oxidize ACN to CN(-) and provide insight for the elucidation of ACN chronic toxicity. |
| Databáze: | OpenAIRE |
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