A Note of Caution: Gramicidin Affects Signaling Pathways Independently of Its Effects on Plasma Membrane Conductance
| Autor: | Julio A. Hernández, Silvia Chifflet, Federico Cabo, Frances Evans |
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| Přispěvatelé: | Evans Isola Frances, Universidad de la República (Uruguay). Facultad de Medicina., Hernández Garrido Julio Andrés, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología., Cabo Federico, Universidad de la República (Uruguay). Facultad de Medicina., Chifflet Silvia, Universidad de la República (Uruguay). Facultad de Medicina. |
| Rok vydání: | 2021 |
| Předmět: |
Article Subject
Membrane lipids Primary Cell Culture Ionophore Microtubules General Biochemistry Genetics and Molecular Biology Ion Channels Membrane Potentials chemistry.chemical_compound Animals Cytoskeleton Membrane potential Ions General Immunology and Microbiology Chemistry Cell Membrane Gramicidin Endothelial Cells Depolarization Epithelial Cells General Medicine Hyperpolarization (biology) Biophysics Plasma Membrane Conductance Medicine Cattle Signal transduction Signal Transduction Research Article |
| Zdroj: | BioMed Research International COLIBRI Universidad de la República instacron:Universidad de la República BioMed Research International, Vol 2021 (2021) |
| ISSN: | 2314-6141 |
| Popis: | Gramicidin is a thoroughly studied cation ionophore widely used to experimentally manipulate the plasma membrane potential (PMP). In addition, it has been established that the drug, due to its hydrophobic nature, is capable of affecting the organization of membrane lipids. We have previously shown that modifications in the plasma membrane potential of epithelial cells in culture determine reorganizations of the cytoskeleton. To elucidate the molecular mechanisms involved, we explored the effects of PMP depolarization on some putative signaling intermediates. In the course of these studies, we came across some results that could not be interpreted in terms of the properties of gramicidin as an ionic channel. The purpose of the present work is to communicate these results and, in general, to draw attention to the fact that gramicidin effects can be misleadingly attributed to its ionic or electrical properties. In addition, this work also contributes with some novel findings of the modifications provoked on the signaling intermediates by PMP depolarization and hyperpolarization. ANII: PR_FCE_2009_1_2474 CSIC: I+D 2014 |
| Databáze: | OpenAIRE |
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