Effect of Yuzu (Citrus junos) Seed Limonoids and Spermine on Intestinal Microbiota and Hypothalamic Tissue in the Sandhoff Disease Mouse Model
| Autor: | Gota Kawai, Tetsuyuki Taniai, Mayumi Minamisawa, Kyoko Suzuki, Shoji Yamanaka, Akira Yamaguchi, Takuma Suzumura, Sudeep Bose |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Limonins
intestinal microbiota Citrus medicine.medical_specialty spermine Normal diet Firmicutes short-chain fatty acids yuzu (Citrus junos) lcsh:Medicine Spermine immunoglobulin A Sandhoff disease Limonoid Article limonoids Mice chemistry.chemical_compound food RNA Ribosomal 16S Internal medicine Lactobacillus Animals Medicine Yuzu (Citrus junos) biology business.industry lcsh:R fungi medicine.disease Citrus junos biology.organism_classification food.food Gastrointestinal Microbiome Disease Models Animal Endocrinology chemistry next-generation sequencing business Dysbiosis medicine.drug |
| Zdroj: | Medical Sciences, Vol 9, Iss 17, p 17 (2021) Medical Sciences Volume 9 Issue 1 |
| ISSN: | 2076-3271 |
| DOI: | 10.3390/medsci9010017 |
| Popis: | The effect of limonoids and spermine (Spm) extracted from yuzu (Citrus junos) seeds on the gut and the brain in a mouse model with Sandhoff disease (SD)was investigated. Wild-type and SD mice were fed a normal diet, or a diet supplemented with limonoid, Spm, or limonoid + Spm for 14–18 weeks, and then 16S rRNA gene amplicon sequencing with extracted DNA from their feces was executed. For SD control mice, intestinal microbiota was mostly composed of Lactobacillus and linked to dysbiosis. For SD and wild-type mice fed with limonoids + Spm or limonoids alone, intestinal microbiota was rich in mucin-degrading bacteria, including Bacteroidetes, Verrucomicrobia, and Firmicutes, and displayed a higher production of short-chain fatty acids and immunoglobulin A. Additionally, SD mice fed with limonoids + Spm or limonoids alone had less inflammation in hypothalamic tissues and displayed a greater number of neurons. Administration of limonoids and/or Spm improved the proportions of beneficial intestinal microbiota to host health and reduced neuronal degeneration in SD mice. Yuzu seed limonoids and Spermine may help to maintain the homeostasis of intestinal microbiota and hypothalamic tissue in the SD mouse model. |
| Databáze: | OpenAIRE |
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