Investigation of the interaction between five alkaloids and human hemoglobin by fluorescence spectroscopy and molecular modeling
| Autor: | Junbiao Chang, Huanjing Dou, Ruiyong Wang, Wu He, Zhigang Li, Lvjing Wang, Xiaogai Wang |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Acefylline
Absorption spectroscopy Molecular model Chemistry Alkaloid Static Electricity Intermolecular force Photochemistry Atomic and Molecular Physics and Optics Fluorescence spectroscopy Analytical Chemistry Molecular Docking Simulation Hemoglobins Alkaloids Spectrometry Fluorescence medicine Humans Thermodynamics Aminophylline Hemoglobin Hydrophobic and Hydrophilic Interactions Instrumentation Spectroscopy Protein Binding medicine.drug |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 123:176-186 |
| ISSN: | 1386-1425 |
| Popis: | This work studied the interaction of human hemoglobin (HHb) with aminophylline, acefylline, caffeine, theophylline and diprophylline systematically by UV–vis absorption spectroscopy and fluorescence spectroscopy in combination with molecular modeling. Five alkaloids caused the fluorescence quenching of HHb by the formation of alkaloids-HHb complex. The binding constants and thermodynamic parameters were obtained. The hydrophobic and electrostatic interactions were the predominant intermolecular forces to stabilize these complexes. Results of thermodynamic analysis and molecular modeling showed that aminophylline was the strongest quencher and diprophylline was the weakest quencher. |
| Databáze: | OpenAIRE |
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