High diversity of multidrug-resistant Mycobacterium tuberculosis Central Asian Strain isolates in Nepal

Autor: Hassan Mahmoud Diab, Chie Nakajima, Ajay Poudel, Norikazu Isoda, Eddie Samuneti Solo, Yogendra Shah, Jeewan Thapa, Bhagwan Maharjan, Basu Dev Pandey, Yasuhiko Suzuki
Rok vydání: 2017
Předmět:
Adult
DNA
Bacterial
Male
0301 basic medicine
Microbiology (medical)
Tuberculosis
Adolescent
Genotyping Techniques
Multidrug-resistant TB
030106 microbiology
Minisatellite Repeats
CAS family
lcsh:Infectious and parasitic diseases
MIRU-VNTR
Mycobacterium tuberculosis
Young Adult
03 medical and health sciences
Bacterial Proteins
Nepal
Drug Resistance
Multiple
Bacterial
Tuberculosis
Multidrug-Resistant
Genotype
medicine
Humans
lcsh:RC109-216
Multidrug-Resistant Mycobacterium tuberculosis
Typing
Child
biology
Infant
Outbreak
DNA-Directed RNA Polymerases
Sequence Analysis
DNA
General Medicine
Middle Aged
biology.organism_classification
rpoB
medicine.disease
Virology
Bacterial Typing Techniques
Multiple drug resistance
030104 developmental biology
Infectious Diseases
Child
Preschool
Female
Public Health
Rifampin
Zdroj: International Journal of Infectious Diseases, Vol 63, Iss C, Pp 13-20 (2017)
ISSN: 1201-9712
DOI: 10.1016/j.ijid.2017.06.010
Popis: Objectives Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB) poses a major public health problem in Nepal. Although it has been reported as one of the dominant genotypes of MTB in Nepal, little information on the Central Asian Strain (CAS) family is available, especially isolates related to multidrug resistance (MDR) cases. This study aimed to elucidate the genetic and epidemiological characteristics of MDR CAS isolates in Nepal. Methods A total of 145 MDR CAS isolates collected in Nepal from 2008 to 2013 were characterized by spoligotyping, mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) analysis, and drug resistance-associated gene sequencing. Results Spoligotyping analysis showed CAS1_Delhi SIT26 as predominant (60/145, 41.4%). However, by combining spoligotyping and MIRU-VNTR typing, it was possible to successfully discriminate all 145 isolates into 116 different types including 18 clusters with 47 isolates (clustering rate 32.4%). About a half of these clustered isolates shared the same genetic and geographical characteristics with other isolates in each cluster, and some of them shared rare point mutations in rpoB that are thought to be associated with rifampicin resistance. Conclusions Although the data obtained show little evidence that large outbreaks of MDR-TB caused by the CAS family have occurred in Nepal, they strongly suggest several MDR-MTB transmission cases.
Databáze: OpenAIRE
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