Synthesis of Poly[d(A-s4T) . d(A-s4T)] by Bacillus subtilis DNA Polymerase
| Autor: | Axel G. Lezius, Ute Rath |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Chemical Phenomena
Stereochemistry DNA polymerase Deoxyribonucleotides Polynucleotides Bacillus subtilis Sulfides Biochemistry chemistry.chemical_compound Deoxyadenosine Adenine nucleotide Escherichia coli Thymine Nucleotides Nucleotide Polymerase chemistry.chemical_classification biology Adenine Nucleotides Templates Genetic biology.organism_classification Chemistry chemistry Polynucleotide DNA Nucleotidyltransferases biology.protein Nucleoside |
| Zdroj: | European Journal of Biochemistry. 24:163-167 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1971.tb19667.x |
| Popis: | Two bacterial DNA polymerases lacking the 5′-exonuclease, isolated from Escherichia coli and Bacillus subtilis are compared with respect to their capability to synthesize the nucleoside analong containing polydeoxynucleotide poly[d(A-s4T) · d(A-s4T)] extensively. Only the enzyme from B. subtilis can use this biopolymer as template for further synthesis, whereas the DNA polymerase A of E. coli requires poly[d(A-T) · d(A-T)] to copy. A method for preparation of pure poly[d(A-s4T) · d(A-s4T)] is described that can be scaled up at will. Proof is given by nucleoside analysis after enzymatic digestion that the polymer consists of equal amounts of deoxyadenosine and 4-thiothymidine nucleotides. Absorption coefficients of poly[d(A-sT) · d(A-s4T)] were determined by two independent methods. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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