Neutrophil activation during acute human anaphylaxis: analysis of MPO and sCD62L
| Autor: | Glenn Arendts, Simon G A Brown, Sally Burrows, Daniel M Fatovich, Yusuf Nagree, Stephen P J Macdonald, Mani Rajee, Erika Bosio, Hugh Mitenko, Shelley F. Stone, Abbie Francis |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Neutrophils Immunology Granulocyte Systemic inflammation Histamine Release Neutrophil Activation 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine medicine Immunology and Allergy Humans Mast Cells L-Selectin Anaphylaxis Peroxidase Innate immune system biology business.industry Allergens Middle Aged medicine.disease Mast cell 030104 developmental biology medicine.anatomical_structure chemistry Myeloperoxidase biology.protein Female Tryptases medicine.symptom business Histamine Biomarkers 030215 immunology Blood sampling |
| Zdroj: | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 47(3) |
| ISSN: | 1365-2222 |
| Popis: | Background The mechanisms involved in the amplification of the mast cell response during anaphylaxis are unclear. Mouse models of anaphylaxis demonstrate the critical involvement of neutrophils. These innate immune cells are highly abundant in peripheral blood and can be rapidly activated to trigger both local and systemic inflammation. Objective To investigate neutrophil activation in peripheral blood during acute human anaphylaxis. Methods Patients presenting to the Emergency Department with anaphylaxis underwent blood sampling upon enrolment and at up to three subsequent time points. Traditional anaphylaxis biomarkers, histamine and mast cell tryptase, were measured by ELISA and ImmunoCAP respectively. Plasma myeloperoxidase concentrations were measured by ELISA, serum soluble CD62L concentrations by cytometric bead array, and both compared to healthy controls. Results In 72 patients, 37 (51%) had severe anaphylaxis, 33 (60%) were histamine positive, and 47 (70%) were mast cell tryptase positive. At enrolment, myeloperoxidase concentrations were 2.9- (95% CI: 1.3, 6.5) and 5.0- (95% CI: 2.4, 10.5) fold higher in moderate and severe patients respectively, compared with healthy controls, and remained stable over the first 5 hours following symptom onset. At enrolment, soluble CD62L was 29% (95% CI: 19, 38) and 31% (95% CI: 22, 40) lower in moderate and severe patients respectively, than healthy controls, and was stable over the first 5 hours. There were no associations between myeloperoxidase or soluble CD62L concentrations and either histamine or mast cell tryptase concentrations. Conclusions and Clinical Relevance These results provide compelling evidence for the involvement of neutrophils during acute human anaphylaxis, suggesting they are activated early in the reaction, regardless of mast cell activation. This important finding increases our understanding of the basic mechanisms of anaphylaxis, a necessary precursor to improving treatment and prevention. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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