Non-cell autonomous requirement for the bloodless gene in primitive hematopoiesis of zebrafish
| Autor: | Leonard I. Zon, Nikolaus S. Trede, Eric C. Liao, Mark W. Kieran, Augustin G. Zapata, David Ransom |
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| Rok vydání: | 2002 |
| Předmět: |
Lymphoid Tissue
education Mutant Apoptosis Bone Morphogenetic Protein 4 Biology Gene product Bone Marrow hemic and lymphatic diseases Proto-Oncogene Proteins medicine Basic Helix-Loop-Helix Transcription Factors Animals GATA1 Transcription Factor Myeloid Cells Molecular Biology Zebrafish T-Cell Acute Lymphocytic Leukemia Protein 1 Genetics Embryogenesis GATA1 Anemia Zebrafish Proteins biology.organism_classification Embryonic stem cell Antigens Differentiation Cell biology Hematopoiesis DNA-Binding Proteins Haematopoiesis medicine.anatomical_structure Bone Morphogenetic Proteins Mutation Erythroid-Specific DNA-Binding Factors Bone marrow Developmental Biology Transcription Factors |
| Zdroj: | Development (Cambridge, England). 129(3) |
| ISSN: | 0950-1991 |
| Popis: | Vertebrate hematopoiesis occurs in two distinct phases, primitive (embryonic) and definitive (adult). Genes that are required specifically for the definitive program, or for both phases of hematopoiesis, have been described. However, a specific regulator of primitive hematopoiesis has yet to be reported. The zebrafish bloodless (bls) mutation causes absence of embryonic erythrocytes in a dominant but incompletely penetrant manner. Primitive macrophages appear to develop normally in bls mutants. Although the thymic epithelium forms normally in bls mutants, lymphoid precursors are absent. Nonetheless, the bloodless mutants can progress through embryogenesis, where red cells begin to accumulate after 5 days post-fertilization (dpf). Lymphocytes also begin to populate the thymic organs by 7.5 dpf. Expression analysis of hematopoietic genes suggests that formation of primitive hematopoietic precursors is deficient in bls mutants and those few blood precursors that are specified fail to differentiate and undergo apoptosis. Overexpression of scl, but not bmp4 or gata1, can lead to partial rescue of embryonic blood cells in bls. Cell transplantation experiments show that cells derived from bls mutant donors can differentiate into blood cells in a wild-type host, but wild-type donor cells fail to form blood in the mutant host. These observations demonstrate that the bls gene product is uniquely required in a non-cell autonomous manner for primitive hematopoiesis, potentially acting via regulation of scl. |
| Databáze: | OpenAIRE |
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