Biorecognition layer engineering: overcoming screening limitations of nanowire-based FET devices
| Autor: | Amir Lichtenstein, Yoni Engel, Larisa Burstein, Raisa Kantaev, Artium Khatchtourints, Fernando Patolsky, Alexander Pevzner, Moria Kwiat, Roey Elnathan |
|---|---|
| Přispěvatelé: | Elnathan, Roey, Kwiat, Moria, Pevzner, Alexander, Engel, Yoni, Burstein, Larisa, Khatchtourints, Artium, Lichtenstein, Amir, Kantaev, Raisa, Patolsky, Fernando |
| Rok vydání: | 2012 |
| Předmět: |
Materials science
Transistors Electronic Silicon dioxide Nanowire Ionic bonding Nanotechnology Bioengineering Biosensing Techniques Microscopy Atomic Force biomolecules chemistry.chemical_compound Immunoglobulin Fab Fragments Troponin T Quantum Dots field effect transistors ionic screening Humans General Materials Science chemistry.chemical_classification antibody fragments Nanowires Mechanical Engineering Biomolecule Osmolar Concentration General Chemistry Blood Proteins Condensed Matter Physics biosensors Silicon Dioxide Chemical species chemistry Ionic strength nanowire Biosensor Layer (electronics) ionic strength Antibodies Immobilized Biomarkers Blood Chemical Analysis |
| Zdroj: | Nano letters. 12(10) |
| ISSN: | 1530-6992 |
| Popis: | Detection of biological species is of great importance to numerous areas of medical and life sciences from the diagnosis of diseases to the discovery of new drugs. Essential to the detection mechanism is the transduction of a signal associated with the specific recognition of biomolecules of interest. Nanowire-based electrical devices have been demonstrated as a powerful sensing platform for the highly sensitive detection of a wide-range of biological and chemical species. Yet, detecting biomolecules in complex biosamples of high ionic strength (>100 mM) is severely hampered by ionic screening effects. As a consequence, most of existing nanowire sensors operate under low ionic strength conditions, requiring ex situ biosample manipulation steps, that is, desalting processes. Here, we demonstrate an effective approach for the direct detection of biomolecules in untreated serum, based on the fragmentation of antibody-capturing units. Size-reduced antibody fragments permit the biorecognition event to occur in closer proximity to the nanowire surface, falling within the charge-sensitive Debye screening length. Furthermore, we explored the effect of antibody surface coverage on the resulting detection sensitivity limit under the high ionic strength conditions tested and found that lower antibody surface densities, in contrary to high antibody surface coverage, leads to devices of greater sensitivities. Thus, the direct and sensitive detection of proteins in untreated serum and blood samples was effectively performed down to the sub-pM concentration range without the requirement of biosamples manipulation. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|