Early Expression of Nucleolar SURF-6 Protein in Mouse Spleen Lymphocytes Activated for Proliferation In Vitro
| Autor: | M. A. Polzikov, Ch. Magoulas, A. A. Moraleva, Olga V. Zatsepina, M. V. Malysheva |
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| Rok vydání: | 2009 |
| Předmět: |
Nucleolus
Lymphocyte Immunoblotting Immunocytochemistry Spleen Lymphocyte Activation General Biochemistry Genetics and Molecular Biology Mice Proliferating Cell Nuclear Antigen Concanavalin A medicine Animals Lymphocytes RRNA processing Cells Cultured Nucleophosmin biology Nuclear Proteins General Medicine Flow Cytometry Immunohistochemistry Molecular biology Proliferating cell nuclear antigen Cell biology medicine.anatomical_structure Gene Expression Regulation biology.protein Mitogens |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 147:578-582 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-009-0578-z |
| Popis: | Using specific antibodies we studied the content of nucleolar SURF-6 protein, which participates in rRNA processing, in mouser spleen lymphocytes activated for proliferation with concanavalin A and compared it with the content of nucleolar nucleophosmin/B23 protein and DNA replication factor PCNA, well-known markers of proliferating cells. Using immunocytochemistry and immunoblotting methods we demonstrate that the concentration of all these proteins increases simultaneously with increasing the proportion of proliferating cells. Unlike nucleophosmin/B23, SURF-6 protein was not revealed in quiescent lymphocyte nucleoli, while the increase of its level in activated lymphocytes preceded elevation of PCNA level. These observations suggest that nucleolar protein SURF-6 can act as a marker of early T lymphocyte activation for proliferation and that it could participate in cell cycle regulation in mammals. |
| Databáze: | OpenAIRE |
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