Multichannel Stroboscopic Videography (MSV): A technique for visualizing multiple channels for behavioral measurements
| Autor: | Theodora Po, Alberto P Soto, Matthew J. McHenry |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Physiology Computer science Water flow 030310 physiology Video Recording Cockroaches Walking Aquatic Science 010603 evolutionary biology 01 natural sciences Pressure field Stroboscope 03 medical and health sciences Behavioral study Water Movements Animals Computer vision Stroboscopy Molecular Biology Swimming Zebrafish Ecology Evolution Behavior and Systematics 0303 health sciences Gromphadorhina portentosa Behavior Animal business.industry Single camera Insect Science Animal Science and Zoology Artificial intelligence Videography business Communication channel |
| Zdroj: | Journal of Experimental Biology. |
| ISSN: | 1477-9145 0022-0949 |
| DOI: | 10.1242/jeb.201749 |
| Popis: | Biologists commonly visualize different features of an organism using distinct sources of illumination. Such multichannel imaging has largely not been applied to behavioral studies because of the challenges posed by a moving subject. We address this challenge with the technique of multichannel stroboscopic videography (MSV), which synchronizes multiple strobe lights with video exposures of a single camera. We illustrate the utility of this approach with kinematic measurements of a walking cockroach ( Gromphadorhina portentosa ) and calculations of the pressure field around a swimming fish ( Danio rerio ). In both, transmitted illumination generated high-contrast images of the animal9s body in one channel. Other sources of illumination were used to visualize the points of contact for the feet of the cockroach and the water flow around the fish in separate channels. MSV provides an enhanced potential for high-throughput experimentation and the capacity to integrate changes in physiological or environmental conditions in freely-behaving animals. |
| Databáze: | OpenAIRE |
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