Trypanosomatid biodiversity in Costa Rica: genotyping of parasites from Heteroptera using the spliced leader RNA gene
| Autor: | R. Zeledón, Dmitri A. Maslov, Nancy R. Sturm, David A. Campbell, Scott J. Westenberger, D. Yanega, Sergei A. Podlipaev |
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| Rok vydání: | 2004 |
| Předmět: |
Costa Rica
RNA Spliced Leader Genotype Molecular Sequence Data Polymerase Chain Reaction Heteroptera Phylogenetics Sequence Homology Nucleic Acid Botany Animals Cluster Analysis Typing Gene Genotyping Phylogeny Base Sequence biology fungi Gene Amplification RNA Biodiversity Exons DNA Protozoan biology.organism_classification Hemiptera DNA extraction Infectious Diseases Evolutionary biology Trypanosomatina Animal Science and Zoology Parasitology Sequence Alignment RNA Protozoan |
| Zdroj: | Parasitology. 129:537-547 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s003118200400592x |
| Popis: | The biodiversity of insect trypanosomes is largely unknown, resulting in significant gaps in the understanding of pathogen evolution. A culture-independent preliminary survey of trypanosomatid fauna was conducted for the parasites of Heteroptera (Hemiptera) from several localities in Costa Rica. Trypanosomatid infections were detected by light microscopy of smeared gut contents. Out of 257 insects representing 6 families, infections were found in 62 cases; cultures were obtained for 29 new isolates. Gut material from infected hosts was preserved in the field using an SDS–EDTA buffer solution for subsequent DNA extraction in the laboratory. PCR amplification of the trypanosomatid-specific spliced leader (SL) RNA gene repeats was successful for 60 field samples. Eighteen distinct SL RNA typing units were identified in a set of 28 samples analysed in detail. Cluster analysis indicated that these typing units were unique and thus could represent new species and, in some cases, new genera. This study reveals only a minor fraction of the trypanosomatid biodiversity, which is anticipated to be high. |
| Databáze: | OpenAIRE |
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