Pinhead signaling regulates mesoderm heterogeneity via FGF receptor-dependent pathway
| Autor: | Aurelian Radu, Jerome Ezan, Olga Ossipova, Sergei Y. Sokol, Keiji Itoh |
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| Rok vydání: | 2020 |
| Předmět: |
Mesoderm
Brachyury animal structures Xenopus Xenopus Proteins Biology Fibroblast growth factor Xenopus laevis 03 medical and health sciences 0302 clinical medicine Transforming Growth Factor beta medicine Animals RNA-Seq Wnt Signaling Pathway Molecular Biology Protein kinase B 030304 developmental biology 0303 health sciences Wnt signaling pathway biology.organism_classification Receptors Fibroblast Growth Factor Cell biology Gastrulation medicine.anatomical_structure embryonic structures NODAL 030217 neurology & neurosurgery Research Article Developmental Biology |
| Zdroj: | Development |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | Among the three embryonic germ layers, mesoderm plays a central role in the establishment of the vertebrate body plan. Mesoderm is specified by secreted signaling proteins from the FGF, Nodal, BMP and Wnt families. No new classes of extracellular mesoderm-inducing factors have been identified in more than two decades. Here we show that the pinhead (pnhd) gene encodes a secreted protein that is essential for the activation of a subset of mesodermal markers in the Xenopus embryo. RNA sequencing revealed that many transcriptional targets of Pnhd are shared with those of the FGF pathway. Pnhd activity was accompanied by Erk phosphorylation and required FGF and Nodal but not Wnt signaling. We propose that during gastrulation Pnhd acts in the marginal zone to contribute to mesoderm heterogeneity via an FGF receptor-dependent positive feedback mechanism. |
| Databáze: | OpenAIRE |
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