ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock
| Autor: | Takehito Tomita, Marina Matsumiya, Ryoichiro Kageyama, Kumiko Yoshioka-Kobayashi, Akihiro Isomura |
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| Rok vydání: | 2018 |
| Předmět: |
Self-organization
0301 basic medicine Cell Cell Culture Techniques Chemical library screening Biology Real-Time Polymerase Chain Reaction Mesoderm Mice 03 medical and health sciences 0302 clinical medicine Biological Clocks Basic Helix-Loop-Helix Transcription Factors Traveling wave Paraxial mesoderm medicine Animals Segmentation Molecular Biology Embryonic Stem Cells In Situ Hybridization Body Patterning Segmentation Clock Synchronized oscillation Oscillation Dynamics (mechanics) Gene Expression Regulation Developmental Stem Cells and Regeneration Embryonic stem cell Cell biology 030104 developmental biology medicine.anatomical_structure Somites Luminescent Measurements Induced presomitic mesoderm Segmentation clock 030217 neurology & neurosurgery Signal Transduction Developmental Biology |
| Zdroj: | Development (Cambridge, England) |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.156836 |
| Popis: | Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7, which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis. This method is applicable to chemical-library screening and will facilitate the analysis of the molecular nature of the segmentation clock. Highlighted Article: Embryonic stem cell-derived presomitic mesoderm-like tissues exhibit synchronized Hes7 oscillations, anterior-posterior-like axis formation and segment formation, and are suitable for chemical-library screening for detailed analysis of the segmentation clock. |
| Databáze: | OpenAIRE |
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