The Impact of TCR Signal Strength on Resident Memory T Cell Formation during Influenza Virus Infection
| Autor: | Sathi Wijeyesinghe, Matthew W. Markman, David Masopust, Marissa Macchietto, Ian A. Stone, Ryan A. Langlois, Steven S. Shen, Jessica K. Fiege, Elizabeth J. Fay |
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| Rok vydání: | 2019 |
| Předmět: |
Immunology
Receptors Antigen T-Cell Heterologous Stimulation Biology medicine.disease_cause Article Virus Epitope Mice 03 medical and health sciences 0302 clinical medicine Orthomyxoviridae Infections T-Lymphocyte Subsets medicine Influenza A virus Animals Immunology and Allergy T-cell receptor Cell Differentiation Phenotype Cell biology Mice Inbred C57BL medicine.anatomical_structure Female Immunologic Memory Memory T cell Signal Transduction 030215 immunology |
| Zdroj: | J Immunol |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Resident memory T cells (TRM) in the lung are vital for heterologous protection against influenza A virus (IAV). Environmental factors are necessary to establish lung TRM; however, the role of T cell–intrinsic factors like TCR signal strength have not been elucidated. In this study, we investigated the impact of TCR signal strength on the generation and maintenance of lung TRM after IAV infection. We inserted high- and low-affinity OT-I epitopes into IAV and infected mice after transfer of OT-I T cells. We uncovered a bias in TRM formation in the lung elicited by lower affinity TCR stimulation. TCR affinity did not impact the overall phenotype or long-term maintenance of lung TRM. Overall, these findings demonstrate that TRM formation is negatively correlated with increased TCR signal strength. Lower affinity cells may have an advantage in forming TRM to ensure diversity in the Ag-specific repertoire in tissues. |
| Databáze: | OpenAIRE |
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