Is auxin involved in the induction of genetic instability in barley homeotic double mutants?
| Autor: | Virginija Vaitkūnienė, Raimondas Šiukšta, Vytautas Rančelis |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genome instability Mutant Plant Science Flowers Biology medicine.disease_cause 01 natural sciences Genomic Instability 03 medical and health sciences Plant Growth Regulators Auxin Genetics medicine Allele Alleles Plant Proteins chemistry.chemical_classification Phenocopy Mutation Indoleacetic Acids Herbicides food and beverages Hordeum 030104 developmental biology Phenotype chemistry Hordeum vulgare 2 4-Dichlorophenoxyacetic Acid Homeotic gene 010606 plant biology & botany |
| Zdroj: | Planta. 247(2) |
| ISSN: | 1432-2048 |
| Popis: | The triggers of genetic instability in barley homeotic double mutants are tweaky spike -type mutations associated with an auxin imbalance in separate spike phytomeres. Barley homeotic tweaky spike;Hooded (tw;Hd) double mutants are characterized by an inherited instability of spike and flower development, which is absent in the single parental constituents. The aim of the present study was to show that the trigger of genetic instability in the double mutants is the tw mutations, which are associated with an auxin imbalance in the developing spikes. Their pleiotropic effects on genes related to spike/flower development may cause the genetic instability of double mutants. The study of four double-mutant groups composed of different mutant alleles showed that the instability arose only if the mutant allele tw was a constituent of the double mutants. Application of auxin inhibitors and 2,4-dichlorophenoxyacetic acid (2,4-D) demonstrated the relationship of the instability of the double mutants and the phenotype of the tw mutants to auxin imbalance. 2,4-D induced phenocopies of the tw mutation in wild-type plants and rescued the phenotypes of three allelic tw mutants. The differential display (dd-PCR) method allowed the identification of several putative candidate genes in tw that may be responsible for the initiation of instability in the double mutants by pleiotropic variations of their expression in the tw mutant associated with auxin imbalance in the developing spikes. The results of the present study linked the genetic instability of homeotic double mutants with an auxin imbalance caused by one of the constituents (tw). The genetic instability of the double mutants in relation to auxin imbalance was studied for the first time. A matrocliny on instability expression was also observed. |
| Databáze: | OpenAIRE |
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