Redefining the H-NS protein family: a diversity of specialized core and accessory forms exhibit hierarchical transcriptional network integration
| Autor: | Daniel M. Stoebel, Stefani C. Kary, Andrew D. S. Cameron, Tzu-Chiao Chao, Ebtihal Y Alshabib, Stephen F. Fitzgerald, Keith D. MacKenzie |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
Protein family AcademicSubjects/SCI00010 Gene regulatory network Repressor Chromatin and Epigenetics Biology Genome 03 medical and health sciences Plasmid Bacterial Proteins Enterobacteriaceae Phylogenetics Genetics Gene Regulatory Networks Gene Phylogeny 030304 developmental biology 0303 health sciences 030306 microbiology Gene regulation Chromatin and Epigenetics Gene Expression Regulation Bacterial Pathogenicity island Gene regulation DNA-Binding Proteins |
| Zdroj: | Fitzgerald, S, Kary, S C, Alshbib, E Y, Mackenzie, K D, Stoebel, D M, Chao, T-C & Cameron, A D S 2020, ' Redefining the H-NS protein family: a diversity of specialized core and accessory forms exhibit hierarchical transcriptional network integration ', Nucleic Acids Research . https://doi.org/10.1093/nar/gkaa709 Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | H-NS is a nucleoid structuring protein and global repressor of virulence and horizontally-acquired genes in bacteria. H-NS can interact with itself or with homologous proteins, but protein family diversity and regulatory network overlap remain poorly defined. Here, we present a comprehensive phylogenetic analysis that revealed deep-branching clades, dispelling the presumption that H-NS is the progenitor of varied molecular backups. Each clade is composed exclusively of either chromosome-encoded or plasmid-encoded proteins. On chromosomes, stpA and newly discovered hlpP are core genes in specific genera, whereas hfp and newly discovered hlpC are sporadically distributed. Six clades of H-NS plasmid proteins (Hpp) exhibit ancient and dedicated associations with plasmids, including three clades with fidelity for plasmid incompatibility groups H, F or X. A proliferation of H-NS homologs in Erwiniaceae includes the first observation of potentially co-dependent H-NS forms. Conversely, the observed diversification of oligomerization domains may facilitate stable co-existence of divergent homologs in a genome. Transcriptomic and proteomic analysis in Salmonella revealed regulatory crosstalk and hierarchical control of H-NS homologs. We also discovered that H-NS is both a repressor and activator of Salmonella Pathogenicity Island 1 gene expression, and both regulatory modes are restored by Sfh (HppH) in the absence of H-NS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|