Comparative Molecular Analysis of Haemophilus influenzae Isolates from Young Children with Acute Lower Respiratory Tract Infections and Meningitis in Hanoi, Vietnam
| Autor: | Olivia Sebastian Rusizoka, Ngo Thi Thi, Kazunori Oishi, Nguyen Thu Nguyet, Hoang Vu Mai Phuong, Hiroshi Watanabe, Vu Thi Lan Huong, Phan Le Thanh Huong, Pham Thi Suu, Tsuyoshi Nagatake, Kiwao Watanabe, Dang Duc Anh, Chiharu Kaji, Nguyen Thi Hien Anh |
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| Rok vydání: | 2005 |
| Předmět: |
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Bacterial Microbiology (medical) Serotype Haemophilus Infections Epidemiology medicine.drug_class Restriction Mapping Antibiotics Biology medicine.disease_cause Meningitis Bacterial Haemophilus influenzae Microbiology medicine Pulsed-field gel electrophoresis Humans Child Respiratory Tract Infections Respiratory tract infections Pasteurellaceae virus diseases Infant medicine.disease biology.organism_classification Antibodies Bacterial Virology Electrophoresis Gel Pulsed-Field Child Preschool Meningitis Bacteria |
| Zdroj: | Journal of Clinical Microbiology. 43:2474-2476 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Thirty-seven Haemophilus influenzae strains from nasopharyngeal swabs (NP) and 44 H. influenzae strains from cerebrospinal fluid (CSF) were investigated. Of the 37 H. influenzae isolates from NP, the serotypes of 30 isolates were nontypeable, 4 were type b, 2 were type c, and 1 was type a, whereas all of the 44 isolates from CSF were type b. The MICs of 16 antibiotics for the H. influenzae isolates from NP and CSF were similar, and no β-lactamase-negative ampicillin-resistant strain was found. Molecular typing by pulsed-field gel electrophoresis (PFGE) showed that the 37 H. influenzae strains from NP had 22 PFGE patterns, with none predominating, and the 44 H. influenzae strains from CSF had 9 PFGE patterns, with patterns α (22 isolates) and β (12 isolates) predominating. Our results indicate that two predominant types of H. influenzae type b strains have the potential to spread among children with meningitis in Hanoi, Vietnam. |
| Databáze: | OpenAIRE |
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