The mouse fas-ligand gene is mutated in gld mice and is part of a TNF family gene cluster
| Autor: | Kent W. Hunter, Mark R. Alderson, Craig A. Smiths, Raymond G. Goodwin, Peter Robert Baum, Terri Davis-Smith, Teresa W. Tough, Deepti Bhat, Wenie S. Din, Marylou Gibson, Michael F. Seldin, Mark L. Watson, Robert E. Miller, David H. Lynch |
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| Rok vydání: | 1994 |
| Předmět: |
Fas Ligand Protein
Genetic Linkage Molecular Sequence Data Immunology Biology Transfection Fas ligand Autoimmune Diseases Cell Line Mice Complementary DNA Gene cluster Animals Point Mutation Immunology and Allergy Amino Acid Sequence Cloning Molecular Gene Southern blot Mice Inbred C3H Membrane Glycoproteins COS cells Base Sequence Tumor Necrosis Factor-alpha Point mutation Chromosome Mapping DNA Molecular biology Mice Inbred C57BL Phenotype Infectious Diseases Multigene Family |
| Zdroj: | Immunity. 1:131-136 |
| ISSN: | 1074-7613 |
| Popis: | The gene for the mouse Fas ligand was cloned and its chromosomal position determined. Fasl was tightly linked to gld (no crossovers in 567 meiotic events) on mouse chromosome 1 and closely linked with a novel member of the same TNF family of ligands, the 0x40 ligand (0 x401 ,1 crossover in 567 meiotic events). Southern blot analysis did not reveal any difference between the Fasl gene from gld and wild-type mice and levels of Fasl mRNA transcripts were similar in PMA and ionomycin induced wild-type and coisogenic gld T cells. Sequence analysis of the gld gene indicated a single amino acid change (Phe Leu) in the COOH terminal portion of this type II transmembrane protein, and COS cells transfected with Fasl cDNA from gld mice failed to induce apoptosis of Fas-expressing target cells. Thus, the data demonstrate that the gld phenotype is the result of a point mutation in the Fasl gene and that Fasl is part of a complex of ligands structurally related to TNF mapping within a small region of mouse chromosome |
| Databáze: | OpenAIRE |
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