Aptameric Recognition-Modulated Electroactivity of Poly(4-Styrenesolfonic Acid)-Doped Polyaniline Films for Single-Shot Detection of Tetrodotoxin
| Autor: | Emmanuel I. Iwuoha, Abd Almonam Baleg, Priscilla G. L. Baker, Gertrude Fomo, Tesfaye Waryo, Christopher E. Sunday |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Polymers
Immobilized Nucleic Acids Analytical chemistry Biosensing Techniques Tetrodotoxin Glassy carbon Electrochemistry lcsh:Chemical technology Biochemistry polyaniline Analytical Chemistry tetrodotoxin sensor chemistry.chemical_compound Limit of Detection Polyaniline Animals lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation Detection limit Aniline Compounds Tetraodontiformes Communication aptamer Electrochemical Techniques Aptamers Nucleotide Atomic and Molecular Physics and Optics Electrochemical gas sensor Dielectric spectroscopy chemistry Linear range Sulfonic Acids Cyclic voltammetry Nuclear chemistry puffer fish |
| Zdroj: | Sensors, Vol 15, Iss 9, Pp 22547-22560 (2015) Sensors (Basel, Switzerland) |
| ISSN: | 1424-8220 |
| Popis: | The work being reported is the first electrochemical sensor for tetrodotoxin (TTX). It was developed on a glassy carbon electrodes (C) that was modified with poly(4-styrenesolfonic acid)-doped polyaniline film (PANI/PSSA). An amine-end functionalized TTX-binding aptamer, 5'-NH₂-AAAAATTTCACACGGGTGCCTCGGCTGTCC-3' (NH₂-Apt), was grafted via covalent glutaraldehyde (glu) cross-linking. The resulting aptasensor (C//PANI⁺/PSSA-glu-NH2-Apt) was interrogated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in sodium acetate buffer (NaOAc, pH 4.8) before and after 30 min incubation in standard TTX solutions. Both CV and EIS results confirmed that the binding of the analyte to the immobilized aptamer modulated the electrochemical properties of the sensor: particularly the charge transfer resistance (Rct) of the PANI⁺/PSSA film, which served as a signal reporter. Based on the Rct calibration curve of the TTX aptasensor, the values of the dynamic linear range (DLR), sensitivity and limit of detection (LOD) of the sensor were determined to be 0.23-1.07 ng·mL(-1) TTX, 134.88 ± 11.42 Ω·ng·mL(-1) and 0.199 ng·mL(-1), respectively. Further studies are being planned to improve the DLR as well as to evaluate selectivity and matrix effects in real samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|