ALDH1A2 (RALDH2) genetic variation in human congenital heart disease
| Autor: | Livia P. Marques, José Eduardo Krieger, Alexandre C. Pereira, Tiago J. P. Sobreira, Viviane F. Ruiz, Fábio Alex Silva, José Xavier-Neto, Marilene Pavan, Paulo Sergio Lopes de Oliveira, Sonia M Mesquita, Antonio Augusto Lopes, Michelle Vasconcelos, Roberta M. Cravo |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Heart Defects
Congenital Protein Folding lcsh:Internal medicine lcsh:QH426-470 Genome-wide association study Single-nucleotide polymorphism Locus (genetics) Biology Polymerase Chain Reaction Polymorphism Single Nucleotide Aldehyde Dehydrogenase 1 Family Linkage Disequilibrium Cell Line ALDH1A2 Exon Research article Genetic variation Genetics Humans Genetic Predisposition to Disease Genetics(clinical) lcsh:RC31-1245 Genetics (clinical) Genetic association Chromosomes Human Pair 15 Genetic Variation Retinal Dehydrogenase Exons Transmission disequilibrium test lcsh:Genetics Mutation Tetralogy of Fallot Genome-Wide Association Study |
| Zdroj: | BMC Medical Genetics, Vol 10, Iss 1, p 113 (2009) BMC Medical Genetics |
| ISSN: | 1471-2350 |
| DOI: | 10.1186/1471-2350-10-113 |
| Popis: | BackgroundSignaling by the vitamin A-derived morphogen retinoic acid (RA) is required at multiple steps of cardiac development. Since conversion of retinaldehyde to RA by retinaldehyde dehydrogenase type II (ALDH1A2, a.k.a RALDH2) is critical for cardiac development, we screened patients with congenital heart disease (CHDs) for genetic variation at the ALDH1A2 locus.MethodsOne-hundred and thirty-three CHD patients were screened for genetic variation at the ALDH1A2 locus through bi-directional sequencing. In addition, six SNPs (rs2704188, rs1441815, rs3784259, rs1530293, rs1899430) at the same locus were studied using a TDT-based association approach in 101 CHD trios. Observed mutations were modeled through molecular mechanics (MM) simulations using the AMBER 9 package, Sander and Pmemd programs. Sequence conservation of observed mutations was evaluated through phylogenetic tree construction from ungapped alignments containing ALDH8 s, ALDH1Ls, ALDH1 s and ALDH2 s. Trees were generated by the Neighbor Joining method. Variations potentially affecting splicing mechanisms were cloned and functional assays were designed to test splicing alterations using the pSPL3 splicing assay.ResultsWe describe in Tetralogy of Fallot (TOF) the mutations Ala151Ser and Ile157Thr that change non-polar to polar residues at exon 4. Exon 4 encodes part of the highly-conserved tetramerization domain, a structural motif required for ALDH oligomerization. Molecular mechanics simulation studies of the two mutations indicate that they hinder tetramerization. We determined that the SNP rs16939660, previously associated with spina bifida and observed in patients with TOF, does not affect splicing. Moreover, association studies performed with classical models and with the transmission disequilibrium test (TDT) design using single marker genotype, or haplotype information do not show differences between cases and controls.ConclusionIn summary, our screen indicates that ALDH1A2 genetic variation is present in TOF patients, suggesting a possible causal role for this gene in rare cases of human CHD, but does not support the hypothesis that variation at the ALDH1A2 locus is a significant modifier of the risk for CHD in humans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|