Mitigated clinical disease in water buffaloes experimentally infected with Babesia bovis
| Autor: | Leonhard Schnittger, María Mesplet, Daniel Benitez, Monica Florin-Christensen, Ignacio Echaide, Susana Torioni de Echaide |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Veterinary medicine Erythrocytes Buffaloes animal diseases 030231 tropical medicine Cattle Diseases Enzyme-Linked Immunosorbent Assay Parasitemia Biology Microbiology Polymerase Chain Reaction Serology 03 medical and health sciences 0302 clinical medicine Babesiosis parasitic diseases medicine Rhipicephalus Animals Serologic Tests Babesia bovis DNA Protozoan medicine.disease biology.organism_classification Breed Immunity Innate 030104 developmental biology Infectious Diseases Hematocrit Insect Science Rhipicephalus microplus Parasitology Cattle Bubalus Nested polymerase chain reaction |
| Zdroj: | Ticks and tick-borne diseases. 9(5) |
| ISSN: | 1877-9603 |
| Popis: | Water buffaloes ( Bubalus bubalis ) are raised in tropical and subtropical regions of the world, and act as hosts of Babesia bovis parasites and the tick vector Rhipicephalus microplus . As no clinical cases of B. bovis- infection have been reported, we hypothesized that, unlike bovines, water buffaloes respond asymptomatically to an acute infection. To test this hypothesis, we inoculated two groups of 24-month-old Mediterranean breed water buffaloes with 10 8 erythrocytes infected with two Argentine B. bovis isolates: BboM2P (n = 5) or BboS2P (n = 5). These strains displayed mild (BboM2P) or high (BboS2P) pathogenicity in Bos taurus calves of the same age (n = 5 and n = 1, respectively), when tested in parallel. In water buffaloes, no changes in body temperature were observed with both strains, and no hematocrit changes were detected in BboM2P-inoculated animals. In contrast, in the BboS2P-inoculated water buffalo group significant but relatively minor reductions in haematocrit values were noted compared to the infected bovine. The parasitemia attained in water buffaloes was considerably lower than in bovines and could only be detected by nested PCR, or indirectly via serology, whereas in most bovines, it could also be detected in Giemsa-stained smears under the light microscope. Our results show that water buffaloes present no or significantly mitigated clinical symptoms to B. bovis infections and suggest that they are able to substantially reduce and/or eliminate B. bovis parasites from circulation by an efficient innate immune mechanism. |
| Databáze: | OpenAIRE |
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