Studies on the Development and Behavior of the Dystrophic Growth Cone, the Hallmark of Regeneration Failure, in anIn VitroModel of the Glial Scar and after Spinal Cord Injury
| Autor: | Catherine Doller, Jerry Silver, Veronica J. Tom, Michael P. Steinmetz, Jared H. Miller |
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| Rok vydání: | 2004 |
| Předmět: |
Neurite
Development/Plasticity/Repair Growth Cones Models Neurological Motility Biology Glial scar Rats Sprague-Dawley Tissue Culture Techniques Cicatrix Dorsal root ganglion In vivo Ganglia Spinal medicine Animals Lectins C-Type Aggrecans Growth cone Spinal cord injury Spinal Cord Injuries Extracellular Matrix Proteins Microscopy Confocal General Neuroscience Regeneration (biology) Dextrans medicine.disease Endocytosis Nerve Regeneration Rats Cell biology Kinetics medicine.anatomical_structure Female Proteoglycans Laminin Synaptic Vesicles Neuroglia Neuroscience |
| Zdroj: | The Journal of Neuroscience. 24:6531-6539 |
| ISSN: | 1529-2401 0270-6474 |
| Popis: | We have developed a novelin vitromodel of the glial scar that mimics the gradient of proteoglycan foundin vivoafter spinal cord injury. In this model, regenerated axons from adult sensory neurons that extended deeply into the gradient developed bulbous, vacuolated endings that looked remarkably similar to dystrophic endings formedin vivo. We demonstrate that despite their highly abnormal appearance and stalled forward progress, dystrophic endings are extremely dynamic bothin vitroandin vivoafter spinal cord injury. Time-lapse movies demonstrated that dystrophic endings continually send out membrane veils and endocytose large membrane vesicles at the leading edge, which were then retrogradely transported to the rear of the “growth cone.” This direction of movement is contrary to membrane dynamics that occur during normal neurite outgrowth. As further evidence of this motility, dystrophic endings endocytosed large amounts of dextran bothin vitroandin vivo. We now have anin vitromodel of the glial scar that may serve as a potent tool for developing and screening potential treatments to help promote regeneration past the lesionin vivo. |
| Databáze: | OpenAIRE |
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