A novel mode of human immunodeficiency virus type 1 (HIV-1) activation: ligation of CD28 alone induces HIV-1 replication in naturally infected lymphocytes
| Autor: | Y. Dudoit, B Asjö, Brigitte Autran, Patrice Debré, D Cefai |
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| Rok vydání: | 1993 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD4-Positive T-Lymphocytes Interleukin 2 CD3 Immunology HIV Infections Receptors Cell Surface In Vitro Techniques Lymphocyte Activation Virus Replication Microbiology Jurkat cells Virus CD28 Antigens Antigens CD Virology medicine Humans biology Cell growth CD28 Viral replication Insect Science HIV-1 biology.protein HIV Long Terminal Repeat Research Article Signal Transduction medicine.drug |
| Zdroj: | Journal of Virology. 67:4395-4398 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Induction of human immunodeficiency virus (HIV) replication in infected CD4+ T lymphocytes requires cellular activation. The ligation of CD28, a signal-transducing receptor with a natural ligand on activated B cells and antigen-presenting cells, provides a costimulating signal for interleukin 2 production and T-cell proliferation as well as coactivation of the transfected HIV long terminal repeat in Jurkat cells. The aim of the present study was to investigate the ability of CD28 ligation to activate HIV type 1 (HIV-1) replication in naturally infected CD4+ lymphocytes either alone or in combination with immobilized anti-CD3 monoclonal antibody. Our results show that HIV-1 was successfully isolated from 16 of 28 patients. For 5 of these 16, virus was isolated only when anti-CD28 was added in combination with the anti-CD3. Moreover, stimulation by anti-CD28 alone induced HIV-1 replication in 5 of 12 patients tested, in the absence of cell proliferation. We found no correlation between the level of CD3- or CD28-induced proliferative response and induction of HIV-1 replication. Therefore, CD28 ligation, a nonmitogenic CD4+ T-cell activation signal, is sufficient to induce transcription and replication of HIV-1 in naturally infected lymphocytes. |
| Databáze: | OpenAIRE |
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