Aquifex aeolicus Trm1[tRNA (m22G26) methyltransferase] has a novel recognition mechanism of the substrate RNA

Autor:	Hiroshi Takeda, Takako Awai, Hiroyuki Hori, Toru Takehara
Rok vydání:	2005
Předmět:	chemistry.chemical_classification tRNA Methyltransferases Aquifex aeolicus Guanine biology RNA General Medicine biology.organism_classification Substrate Specificity Gene product Enzyme Bacterial Proteins RNA Transfer chemistry Biochemistry Transfer RNA Protein biosynthesis Eubacterium Gene
Zdroj:	Nucleic Acids Symposium Series. 49:303-304
ISSN:	1746-8272 0261-3166
DOI:	10.1093/nass/49.1.303
Popis:	Aquifex aeolicus is a hyper-thermophilic eubacterium, which probably diverged at the earliest period from the other bacteria on the evolution of life. Therefore, analyses of RNA modification enzymes of this bacterium may supply important information in relation to the establishment of the early protein synthesis. In the previous meeting, we have reported that Aquifex aeolicus trm1 gene product possesses a tRNA (m2(2)G26) methyltransferase activity. This enzyme catalyzes the methyl-transfer from S-adenosyl-L-methionine to the semi-conserved residue, G26, via the intermediate modified base, m2G26. In this meeting, we report the A. aeolicus Trm1 large scale expression system in E. coli and the substrate RNA recognition mechanism of the enzyme.
Databáze:	OpenAIRE
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