Genetic and agronomic traits stability of marker-free transgenic wheat plants generated from Agrobacterium-mediated co-transformation in T2 and T3 generations
| Autor: | Jing Wang, Ke Wang, Xing-guo Ye, Li-pu Du, Huiyun Liu, Xinwu Pei |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
marker-free transgenic plants Agrobacterium Transgene Agriculture (General) Plant Science Genetically modified crops Genetically modified wheat Biology 01 natural sciences Biochemistry S1-972 Biosafety Food Animals wheat genetic stability Gene fluorescence in situ hybridization Ecology business.industry food and beverages 04 agricultural and veterinary sciences biology.organism_classification Biotechnology Transformation (genetics) Agriculture 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Animal Science and Zoology business Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 19, Iss 1, Pp 23-32 (2020) |
| ISSN: | 2095-3119 |
| Popis: | Genetically modified wheat has not been commercially utilized in agriculture largely due to regulatory hurdles associated with traditional transformation methods. Development of marker-free transgenic wheat plants will help to facilitate biosafety evaluation and the eventual environmental release of transgenic wheat varieties. In this study, the marker-free transgenic wheat plants previously obtained by Agrobacterium-mediated co-transformation of double T-DNAs vector were identified by fluorescence in situ hybridization (FISH) in the T1 generation, and their genetic stability and agronomic traits were analyzed in T2 and T3 generations. FISH analysis indicated that the transgene often integrated into a position at the distal region of wheat chromosomes. Furthermore, we show that the GUS transgene was stably inherited in the marker-free transgenic plants in T1 to T3 generations. No significant differences in agronomic traits or grain characteristics were observed in T3 generation, with the exception of a small variation in spike length and grains per spike in a few lines. The selection marker of bar gene was not found in the transgenic plants through T1 to T3 generations. The results from this investigation lay a solid foundation for the potential application of the marker-free transgenic wheat plants achieved through the co-transformation of double T-DNAs vector by Agrobacterium in agriculture after biosafty evaluation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect